Abstract

The overall goal of the research is to understand the developmental processes that take place in Chip embryonic limb. In this developmental system, changes occur in the activity of a number of genes at the time of differentiation of limb mesodermal cells into chondrocytes. We will study: a) the structure-function relationship of the translation products of newly activated genes in the establishment of the cartilaginous matrix, b) the regulatory sequences that are specific for the expression of one of these genes in chondrocytes and c) the morphogenetic consequences of induced and spontaneous mutations that affect cartilage matrix macromolecules.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD022016-11
Application #
2198406
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1986-01-01
Project End
1996-02-29
Budget Start
1994-03-01
Budget End
1996-02-29
Support Year
11
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Baciu, P C; Saoncella, S; Lee, S H et al. (2000) Syndesmos, a protein that interacts with the cytoplasmic domain of syndecan-4, mediates cell spreading and actin cytoskeletal organization. J Cell Sci 113 Pt 2:315-24
Saoncella, S; Echtermeyer, F; Denhez, F et al. (1999) Syndecan-4 signals cooperatively with integrins in a Rho-dependent manner in the assembly of focal adhesions and actin stress fibers. Proc Natl Acad Sci U S A 96:2805-10
Huang, X; Birk, D E; Goetinck, P F (1999) Mice lacking matrilin-1 (cartilage matrix protein) have alterations in type II collagen fibrillogenesis and fibril organization. Dev Dyn 216:434-41
Echtermeyer, F; Baciu, P C; Saoncella, S et al. (1999) Syndecan-4 core protein is sufficient for the assembly of focal adhesions and actin stress fibers. J Cell Sci 112 ( Pt 20):3433-41
Hofer, U; Kahoussi, B; Trelstad, J et al. (1996) Expression of functional link protein domains using an avian-specific retroviral vector. Ann N Y Acad Sci 785:271-3
Yang, B; Yang, B L; Goetinck, P F (1996) Construction and expression of a functional recombinant gene for proteoglycan. Ann N Y Acad Sci 785:356-9
Chen, Q; Johnson, D M; Haudenschild, D R et al. (1996) Cartilage matrix protein: expression patterns in chicken, mouse, and human. Ann N Y Acad Sci 785:238-40
Yang, B; Yang, B L; Goetinck, P F (1995) Biotinylated hyaluronic acid as a probe for identifying hyaluronic acid-binding proteins. Anal Biochem 228:299-306
Haudenschild, D R; Tondravi, M M; Hofer, U et al. (1995) The role of coiled-coil alpha-helices and disulfide bonds in the assembly and stabilization of cartilage matrix protein subunits. A mutational analysis. J Biol Chem 270:23150-4
Baciu, P C; Goetinck, P F (1995) Protein kinase C regulates the recruitment of syndecan-4 into focal contacts. Mol Biol Cell 6:1503-13

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