Abstract

This application focuses upon the role of oocytes in the development of heterogeneous patterns of gene expression by different types of granulosa cells after follicular antrum formation. The investigator hypothesizes that oocytes promote the development of the cumulus cell phenotype by both suppressing and inducing expression of cell-specific transcripts.
The Specific aims are to test: 1) the hypothesis that mouse oocytes promote expression of IGF-1 mRNA in granulosa cells of preantral follicles and cumulus cells of preovulatory follicles; 2) the hypothesis that oocytes from preovulatory follicles, but not those from preantral follicles, promote the expression of cumulus cell-specific transcripts; 3) whether oocytes from preantral follicles and antral follicles have different effects on kit ligand (KL) mRNA expression by granulosa cells and whether these different effects depend upon whether the granulosa cells are from preantral or antral follicles; and 4) whether species-specific patterns of granulosa cell gene expression are determined by oocytes in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD023839-11
Application #
6138761
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Tasca, Richard J
Project Start
1988-02-03
Project End
2002-12-31
Budget Start
2000-01-01
Budget End
2000-12-31
Support Year
11
Fiscal Year
2000
Total Cost
$312,412
Indirect Cost

  Institution

Name
Jackson Laboratory
Department
Type
DUNS #
042140483
City
Bar Harbor
State
ME
Country
United States
Zip Code
04609

  Publications

Guo, Jing; Shi, Lanying; Gong, Xuhong et al. (2016) Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus-oocyte complexes. J Cell Sci 129:3091-103
Wigglesworth, Karen; Lee, Kyung-Bon; Emori, Chihiro et al. (2015) Transcriptomic diversification of developing cumulus and mural granulosa cells in mouse ovarian follicles. Biol Reprod 92:23
Peng, Jia; Wigglesworth, Karen; Rangarajan, Adithya et al. (2014) Amino acid 72 of mouse and human GDF9 mature domain is responsible for altered homodimer bioactivities but has subtle effects on GDF9:BMP15 heterodimer activities. Biol Reprod 91:142
Lee, Kyung-Bon; Zhang, Meijia; Sugiura, Koji et al. (2013) Hormonal coordination of natriuretic peptide type C and natriuretic peptide receptor 3 expression in mouse granulosa cells. Biol Reprod 88:42
Wigglesworth, Karen; Lee, Kyung-Bon; O'Brien, Marilyn J et al. (2013) Bidirectional communication between oocytes and ovarian follicular somatic cells is required for meiotic arrest of mammalian oocytes. Proc Natl Acad Sci U S A 110:E3723-9
Dokshin, Gregoriy A; Baltus, Andrew E; Eppig, John J et al. (2013) Oocyte differentiation is genetically dissociable from meiosis in mice. Nat Genet 45:877-83
Peng, Jia; Li, Qinglei; Wigglesworth, Karen et al. (2013) Growth differentiation factor 9:bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions. Proc Natl Acad Sci U S A 110:E776-85
Peng, Jia; Li, Qinglei; Wigglesworth, Karen et al. (2013) Reply to Mottershead et al.: GDF9:BMP15 heterodimers are potent regulators of ovarian functions. Proc Natl Acad Sci U S A 110:E2258
Emori, Chihiro; Wigglesworth, Karen; Fujii, Wataru et al. (2013) Cooperative effects of 17?-estradiol and oocyte-derived paracrine factors on the transcriptome of mouse cumulus cells. Endocrinology 154:4859-72
Zhang, Meijia; Su, You-Qiang; Sugiura, Koji et al. (2011) Estradiol promotes and maintains cumulus cell expression of natriuretic peptide receptor 2 (NPR2) and meiotic arrest in mouse oocytes in vitro. Endocrinology 152:4377-85

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