The long term objective of these studies is to decode the genetic information contained within a relatively small chromosomal region in the central portion of mouse chromosome 5. Classical genetic studies have resulted in the identification of several developmental mutations (W, Ph, Rw, rs, l, pi) within this region. Recent studies have shown that two of these mutations (Ph and W) are caused by alterations in genes encoding receptor tyrosine kinases. The extensive information about the genetic structure of this region, and its involvement in key developmental processes, provide a rationale for the establishment of a more comprehensive functional map. The experiments proposed here will provide both a structural map consisting of cloned and characterized mutation breakpoints associated with chromosomal rearrangement and a transcription map consisting of an array of partially characterized physically mapped exons/genes. To define the functional role of the genes in this region, a hemizygous screen for novel visible / lethal / behavioral mutations among the progeny of chemically mutagenized mice will also be performed. Complementation analysis and phenotypic characterization of new and several preexisting mutations in this region will result in significant progress toward the long term goal of these studies - to determine the relationship between genes defined by mutations, molecularly defined transcription units and gene sequences identified by large scale genomic sequencing. Molecular genetic information about the function of genes within the central portion of mouse chromosome 5 will be directly applicable to the genetic dissection of the homologous region on human chromosome 4. Moreover, the results of this project will facilitate a global study of the mammalian genome and provide insight into genome alterations that lead to abnormal function / disease.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD028410-10
Application #
6182299
Study Section
Mammalian Genetics Study Section (MGN)
Program Officer
Moody, Sally Ann
Project Start
1991-07-01
Project End
2001-11-30
Budget Start
2000-07-01
Budget End
2001-11-30
Support Year
10
Fiscal Year
2000
Total Cost
$470,719
Indirect Cost
Name
University of Pennsylvania
Department
Genetics
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Crabtree, J; Wiltshire, T; Brunk, B et al. (2001) High-resolution BAC-based map of the central portion of mouse chromosome 5. Genome Res 11:1746-57
Alavizadeh, A; Kiernan, A E; Nolan, P et al. (2001) The Wheels mutation in the mouse causes vascular, hindbrain, and inner ear defects. Dev Biol 234:244-60
Hurle, B; Lane, K; Kenney, J et al. (2001) Physical mapping of the mouse tilted locus identifies an association between human deafness loci DFNA6/14 and vestibular system development. Genomics 77:189-99
Tarantino, L M; Feiner, L; Alavizadeh, A et al. (2000) A high-resolution radiation hybrid map of the proximal portion of mouse chromosome 5. Genomics 66:55-64
Lengeling, A; Wiltshire, T; Otmani, C et al. (1999) A sequence-ready BAC contig of the GABAA receptor gene cluster Gabrg1-Gabra2-Gabrb1 on mouse chromosome 5. Genome Res 9:732-8
Hough, R B; Lengeling, A; Bedian, V et al. (1998) Rump white inversion in the mouse disrupts dipeptidyl aminopeptidase-like protein 6 and causes dysregulation of Kit expression. Proc Natl Acad Sci U S A 95:13800-5
Schimenti, J; Bucan, M (1998) Functional genomics in the mouse: phenotype-based mutagenesis screens. Genome Res 8:698-710
Zimmerman, J E; Bui, Q T; Steingrimsson, E et al. (1997) Cloning and characterization of two vertebrate homologs of the Drosophila eyes absent gene. Genome Res 7:128-41
Kluppel, M; Nagle, D L; Bucan, M et al. (1997) Long-range genomic rearrangements upstream of Kit dysregulate the developmental pattern of Kit expression in W57 and Wbanded mice and interfere with distinct steps in melanocyte development. Development 124:65-77
Nolan, P M; Kapfhamer, D; Bucan, M (1997) Random mutagenesis screen for dominant behavioral mutations in mice. Methods 13:379-95

Showing the most recent 10 out of 22 publications