Abstract

The ultimate objective of this work is to understand how receptors and other DNA-binding proteins interact to regulate the transcription of tissue- and hormone-specific genes.
The aim of this proposal is to understand how PRL augments the progesterone-dependent transcription of the UG gene by regulating as many as four proteins that bind to the UG promoter. This goal will be achieved by 1) cloning and characterizing the cDNAs for PRL/progesterone-dependent proteins that bind to the UG promoter; 2) determining whether PRL, in combination with progesterone, regulates the sequence-specific binding activity of the UG promoter binding (UGPB) proteins; 3) quantifying hormone-dependent changes in the expression of mRNA for UGPB proteins by specific uterine cell types; 4) characterizing the interactions of PRL and progesterone in the regulation of the UG promoter in an in vitro cell transfection system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
1R01HD029457-01A2
Application #
2201881
Study Section
Reproductive Endocrinology Study Section (REN)
Project Start
1993-12-01
Project End
1996-11-30
Budget Start
1993-12-01
Budget End
1994-11-30
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Texas Tech University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
609980727
City
Lubbock
State
TX
Country
United States
Zip Code
79430

  Publications

Helmer, Rebecca A; Panchoo, Marlyn; Dertien, Janet S et al. (2010) Prolactin-induced Jak2 phosphorylation of RUSH: a key element in Jak/RUSH signaling. Mol Cell Endocrinol 325:143-9
Hewetson, Aveline; Wright-Pastusek, Amber E; Helmer, Rebecca A et al. (2008) Conservation of inter-protein binding sites in RUSH and RFBP, an ATP11B isoform. Mol Cell Endocrinol 292:79-86
Hewetson, Aveline; Chilton, Beverly S (2008) Progesterone-dependent deoxyribonucleic acid looping between RUSH/SMARCA3 and Egr-1 mediates repression by c-Rel. Mol Endocrinol 22:813-22
Chilton, Beverly S; Hewetson, Aveline (2008) Progesterone regulation of RUSH/SMARCA3/HLTF includes DNA looping. Biochem Soc Trans 36:632-6
Mukherjee, Anil B; Zhang, Zhongjian; Chilton, Beverly S (2007) Uteroglobin: a steroid-inducible immunomodulatory protein that founded the Secretoglobin superfamily. Endocr Rev 28:707-25
Chilton, Beverly S; Hewetson, Aveline (2005) Prolactin and growth hormone signaling. Curr Top Dev Biol 68:1-23
Hewetson, Aveline; Moore, Shelli L; Chilton, Beverly S (2004) Prolactin signals through RUSH/SMARCA3 in the absence of a physical association with Stat5a. Biol Reprod 71:1907-12
Hewetson, Aveline; Chilton, Beverly S (2003) An Sp1-NF-Y/progesterone receptor DNA binding-dependent mechanism regulates progesterone-induced transcriptional activation of the rabbit RUSH/SMARCA3 gene. J Biol Chem 278:40177-85
Mansharamani, Malini; Chilton, Beverly S (2002) The reproductive importance of P-type ATPases. Mol Cell Endocrinol 188:22-5
Hewetson, Aveline; Hendrix, Ericka C; Mansharamani, Malini et al. (2002) Identification of the RUSH consensus-binding site by cyclic amplification and selection of targets: demonstration that RUSH mediates the ability of prolactin to augment progesterone-dependent gene expression. Mol Endocrinol 16:2101-12

Showing the most recent 10 out of 21 publications