Heterochromatin is a major component of higher eukaryotic genomes and plays an important role in chromosomes and cell biology. Errors in chromosome inheritance and rearrangements involving heterochromatin are associated with a variety of human disorders. The knowledge base of heterochromatin structure and function is limited due to the lack of detailed molecular information about this important genomic component. A 1 Mb heterochromatic region of the 1.3 Mb Drosophila minichromosome Dp1187 represents a model for heterochromatin studies.
The aims of this proposal are to further characterize this region as an extension of previous studies. They include the following: 1) complete the sequence analysis of the 420 kb centromere, a key component of chromosome inheritance in all eukaryotes; 2) generate tools and information to initiate the mapping and sequence analysis of many other regions of Drosophila heterochromatin, and 3) collaborate with FlyBase, a Drosophila internet database resource, to collect and integrate information about Drosophila heterochromatin.
| Hoskins, Roger A; Carlson, Joseph W; Wan, Kenneth H et al. (2015) The Release 6 reference sequence of the Drosophila melanogaster genome. Genome Res 25:445-58 |
| Werren, John H; Richards, Stephen; Desjardins, Christopher A et al. (2010) Functional and evolutionary insights from the genomes of three parasitoid Nasonia species. Science 327:343-8 |
| Sharakhova, Maria V; George, Phillip; Brusentsova, Irina V et al. (2010) Genome mapping and characterization of the Anopheles gambiae heterochromatin. BMC Genomics 11:459 |
| Xia, Ai; Sharakhova, Maria V; Leman, Scotland C et al. (2010) Genome landscape and evolutionary plasticity of chromosomes in malaria mosquitoes. PLoS One 5:e10592 |
| Hoang, Margaret L; Tan, Frederick J; Lai, David C et al. (2010) Competitive repair by naturally dispersed repetitive DNA during non-allelic homologous recombination. PLoS Genet 6:e1001228 |
| Karpen, Gary H (2009) Preparation of high-molecular-weight DNA from Drosophila embryos. Cold Spring Harb Protoc 2009:pdb.prot5254 |
| Peng, Jamy C; Karpen, Gary H (2008) Epigenetic regulation of heterochromatic DNA stability. Curr Opin Genet Dev 18:204-11 |
| Smith, Christopher D; Edgar, Robert C; Yandell, Mark D et al. (2007) Improved repeat identification and masking in Dipterans. Gene 389:1-9 |
| Hoskins, Roger A; Carlson, Joseph W; Kennedy, Cameron et al. (2007) Sequence finishing and mapping of Drosophila melanogaster heterochromatin. Science 316:1625-8 |
| (2007) Evolution of genes and genomes on the Drosophila phylogeny. Nature 450:203-18 |
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