We will study the influence of four dietary factors, namely cholesterol, ethanol, lecithin, and unsaturated versus saturated fat, on plasma lipoprotein, and lipid distribution and concentration. To determine dietary effects on lipoprotein structure, measurements of physical properties and chemical composition will be carried out. Experiments have been planned in factorial design so that the counterbalancing or synergistic interactions among the dietary ingredients that affect lipoprotein structure and concentration will be learned. Dietary ingredients to be studied were selected to maximize variability in HDL response. African green monkeys will be used as animal models because of their known similarities to human beings in lipoprotein response to diet, and to permit metabolic studies of HDL formation and catabolism. Thoracic lymph duct cannulated animals will be used to study dietary effects on several aspects of HDL formation in the intestine. These studies were designed to test the hypothesis that dietary factors influence the rate and amount of production, the chemical composition, and the physical properties of the precursor lipoproteins of the intestine, (primarily the chylomicra) which are then reflected in the properties, turnover rates, and eventual concentrations of HDL. At the completion of the study, some of the animals will be killed and the extent and severity of coronary artery atherosclerosis will be measured. Relationships between diet, lipoproteins, and atherosclerosis among the same individuals can then be used to establish the atherogenic features of diet-induced hyperlipoproteinemia.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
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Pathology A Study Section (PTHA)
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Wake Forest University Health Sciences
Schools of Medicine
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Kavanagh, Kylie; Wylie, Ashley T; Chavanne, Tara J et al. (2012) Aging does not reduce heat shock protein 70 in the absence of chronic insulin resistance. J Gerontol A Biol Sci Med Sci 67:1014-21
Sacks, Frank M; Rudel, Lawrence L; Conner, Adam et al. (2009) Selective delipidation of plasma HDL enhances reverse cholesterol transport in vivo. J Lipid Res 50:894-907
Degirolamo, Chiara; Shelness, Gregory S; Rudel, Lawrence L (2009) LDL cholesteryl oleate as a predictor for atherosclerosis: evidence from human and animal studies on dietary fat. J Lipid Res 50 Suppl:S434-9
Parini, Paolo; Gustafsson, Ulf; Davis, Matt A et al. (2008) Cholesterol synthesis inhibition elicits an integrated molecular response in human livers including decreased ACAT2. Arterioscler Thromb Vasc Biol 28:1200-6
Baiga, Thomas J; Guo, Haibing; Xing, Yalan et al. (2008) Metabolite induction of Caenorhabditis elegans dauer larvae arises via transport in the pharynx. ACS Chem Biol 3:294-304
Chao, Yi-Chun E; Zhao, Yue; Kupper, Lawrence L et al. (2008) Quantifying the relative importance of predictors in multiple linear regression analyses for public health studies. J Occup Environ Hyg 5:519-29
Kavanagh, Kylie; Jones, Kate L; Sawyer, Janet et al. (2007) Trans fat diet induces abdominal obesity and changes in insulin sensitivity in monkeys. Obesity (Silver Spring) 15:1675-84
Temel, Ryan E; Hou, Li; Rudel, Lawrence L et al. (2007) ACAT2 stimulates cholesteryl ester secretion in apoB-containing lipoproteins. J Lipid Res 48:1618-27
Bell 3rd, Thomas A; Wilson, Martha D; Kelley, Kathryn et al. (2007) Monounsaturated fatty acyl-coenzyme A is predictive of atherosclerosis in human apoB-100 transgenic, LDLr-/- mice. J Lipid Res 48:1122-31
Wallace, Jeanne M; Schwarz, Margrit; Coward, Peter et al. (2005) Effects of peroxisome proliferator-activated receptor alpha/delta agonists on HDL-cholesterol in vervet monkeys. J Lipid Res 46:1009-16

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