Abstract

Current progress from this laboratory has indicated that endotoxin administration impairs myocardial metabolism by two different mechanisms: one by acting through membrane lipid microenvironment and the other by affecting protein phosphorylation/dephosphorylation. The primary aims of this proposal are to continue our search for the understanding of pathophysiology of myocardial dysfunction at the cellular level and, ultimately, to propose a rational remedy for the better management of shock. The specific objectives include studies of phosphorylaton/dephosphorylation of cardiac sarcolemma and sarcoplasmic reticulum in relationship to membrane enzyme and receptor dynamics; investigation of phosphorylation/dephosphorylation of contractile proteins and its association with myofibrillar ATPase activities; studies of the biological activity of calmodulin and the Ca2+ binding properties of calmodulin and triponin C; analysis of the chemical structure and physical properties of myocyte membrane lipids; studies of myocyte-liposome interactions; and development of a liposome preparation to be used as a membrane modifier and as a drug carrier system for the therapeutic interventions of endotoxin shock.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL030080-05
Application #
3341158
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1982-07-01
Project End
1990-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
5
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Saint Louis University
Department
Type
Schools of Medicine
DUNS #
City
Saint Louis
State
MO
Country
United States
Zip Code
63103

  Publications

Yang, Rei-Cheng; Hsu, Chin; Lee, Tzu-Ying et al. (2013) Transcriptional Regulation of the Group IIA Secretory Phospholipase A2 Gene by C/EBP? in Rat liver and its Relationship to Hepatic Gluconeogenesis during Sepsis. Emerg Med (Los Angel) 3:151
Liu, Maw-Shung; Yang, Rei-Cheng; Hsu, Chin et al. (2013) Changes in group II phospholipase A2 gene expression in rat heart during sepsis. J Surg Res 181:272-8
Hsu, Chin; Wu, Guang; Yang, Shaw-Lang et al. (2007) Intracellular redistribution of dihydropyridine receptor in the rat heart during the progression of sepsis. J Surg Res 141:146-52
Wu, Guang; Yang, Shaw-Lang; Hsu, Chin et al. (2004) Transcriptional regulation of cardiac sarcoplasmic reticulum calcium-ATPase gene during the progression of sepsis. Shock 22:46-50
Wu, Li-Ling; Yang, Shaw-Lang; Yang, Rei-Cheng et al. (2003) G protein and adenylate cyclase complex-mediated signal transduction in the rat heart during sepsis. Shock 19:533-7
Wu, Li-Ling; Tang, Chaoshu; Dong, Lin-Wang et al. (2002) Altered phospholamban-calcium ATPase interaction in cardiac sarcoplasmic reticulum during the progression of sepsis. Shock 17:389-93
Dong, L W; Wu, L L; Ji, Y et al. (2001) Impairment of the ryanodine-sensitive calcium release channels in the cardiac sarcoplasmic reticulum and its underlying mechanism during the hypodynamic phase of sepsis. Shock 16:33-9
Wu, L L; Tang, C; Liu, M S (2001) Altered phosphorylation and calcium sensitivity of cardiac myofibrillar proteins during sepsis. Am J Physiol Regul Integr Comp Physiol 281:R408-16
Wu, L L; Ji, Y; Dong, L W et al. (2001) Calcium uptake by sarcoplasmic reticulum is impaired during the hypodynamic phase of sepsis in the rat heart. Shock 15:49-55
Dong, L W; Tang, C; Liu, M S (2000) Biphasic redistribution of muscarinic receptor and the altered receptor phosphorylation and gene transcription are underlying mechanisms in the rat heart during sepsis. Cardiovasc Res 45:925-33

Showing the most recent 10 out of 49 publications