Abstract

The proposed experiments will describe the molecular processes by which angiotensin II (AII) regulates transcription of the renin gene. The first specific aim will characterize the in vivo responsiveness of renin transcription to AII infusion. These studies will entail an assessment of renin transcription in salt-depleted and salt replete rats following infusion of either AII, ACE inhibitor or both. The results will then be correlated to changes in renin release as measured by PRA.
Specific aims 2 and 3 will assess the molecular basis for the acute and chronic regulation of renin expression by AII, respectively. These experiments will identify and characterize the modulation of putative transcriptional regulatory proteins which influence renin expression. Finally, specific aim 4 will entail cloning of these transcriptional regulatory factors. The cDNAs thus isolated will then be used for expression surveys. These studies should further elucidate the molecular events that govern regulation of renin expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL035610-08
Application #
3349615
Study Section
Experimental Cardiovascular Sciences Study Section (ECS)
Project Start
1985-09-30
Project End
1994-03-30
Budget Start
1992-04-01
Budget End
1993-03-31
Support Year
8
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Hodgkinson, Conrad P; Gomez, José A; Baksh, Syeda Samara et al. (2018) Insights from molecular signature of in vivo cardiac c-Kit(+) cells following cardiac injury and ?-catenin inhibition. J Mol Cell Cardiol 123:64-74
Matsushita, Kenichi; Wu, Yaojiong; Pratt, Richard E et al. (2016) Deletion of angiotensin II type 2 receptor accelerates adipogenesis in murine mesenchymal stem cells via Wnt10b/beta-catenin signaling. Lab Invest 96:909-17
Matsushita, Kenichi; Morello, Fulvio; Zhang, Zhiping et al. (2016) Nuclear hormone receptor LXR? inhibits adipocyte differentiation of mesenchymal stem cells with Wnt/beta-catenin signaling. Lab Invest 96:230-8
Yuan, Hsiangkuo; Gomez, Jose A; Chien, Jennifer S et al. (2016) Tracking mesenchymal stromal cells using an ultra-bright TAT-functionalized plasmonic-active nanoplatform. J Biophotonics 9:406-13
Yang, Yanqiang; Gomez, Jose A; Herrera, Marcela et al. (2015) Salt restriction leads to activation of adult renal mesenchymal stromal cell-like cells via prostaglandin E2 and E-prostanoid receptor 4. Hypertension 65:1047-54
Schmeckpeper, Jeffrey; Verma, Amanda; Yin, Lucy et al. (2015) Inhibition of Wnt6 by Sfrp2 regulates adult cardiac progenitor cell differentiation by differential modulation of Wnt pathways. J Mol Cell Cardiol 85:215-25
Huang, Jing; Guo, Jian; Beigi, Farideh et al. (2014) HASF is a stem cell paracrine factor that activates PKC epsilon mediated cytoprotection. J Mol Cell Cardiol 66:157-64
Hodgkinson, Conrad P; Gomez, Jose A; Payne, Alan J et al. (2014) Abi3bp regulates cardiac progenitor cell proliferation and differentiation. Circ Res 115:1007-16
Beigi, Farideh; Schmeckpeper, Jeffrey; Pow-Anpongkul, Pete et al. (2013) C3orf58, a novel paracrine protein, stimulates cardiomyocyte cell-cycle progression through the PI3K-AKT-CDK7 pathway. Circ Res 113:372-80
Wang, Hao; Gomez, Jose A; Klein, Sabine et al. (2013) Adult renal mesenchymal stem cell-like cells contribute to juxtaglomerular cell recruitment. J Am Soc Nephrol 24:1263-73

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