Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL048318-05
Application #
2224385
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1992-04-01
Project End
1997-07-31
Budget Start
1996-04-01
Budget End
1997-07-31
Support Year
5
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Parker, Christine H; Morgan, Christopher R; Rand, Kasper D et al. (2014) A conformational investigation of propeptide binding to the integral membrane protein ?-glutamyl carboxylase using nanodisc hydrogen exchange mass spectrometry. Biochemistry 53:1511-20
Tie, J-K; Jin, D-Y; Tie, K et al. (2013) Evaluation of warfarin resistance using transcription activator-like effector nucleases-mediated vitamin K epoxide reductase knockout HEK293 cells. J Thromb Haemost 11:1556-64
Tie, Jian-Ke; Jin, Da-Yun; Stafford, Darrel W (2012) Human vitamin K epoxide reductase and its bacterial homologue have different membrane topologies and reaction mechanisms. J Biol Chem 287:33945-55
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Higgins-Gruber, Shannon L; Mutucumarana, Vasantha P; Lin, Pen-Jen et al. (2010) Effect of vitamin K-dependent protein precursor propeptide, vitamin K hydroquinone, and glutamate substrate binding on the structure and function of {gamma}-glutamyl carboxylase. J Biol Chem 285:31502-8
Hebling, Christine M; Morgan, Christopher R; Stafford, Darrel W et al. (2010) Conformational analysis of membrane proteins in phospholipid bilayer nanodiscs by hydrogen exchange mass spectrometry. Anal Chem 82:5415-9
Tie, Jian-Ke; Zheng, Mei-Yan; Hsiao, Kuang-Ling N et al. (2008) Transmembrane domain interactions and residue proline 378 are essential for proper structure, especially disulfide bond formation, in the human vitamin K-dependent gamma-glutamyl carboxylase. Biochemistry 47:6301-10
Gui, Tong; Reheman, Adili; Funkhouser, William K et al. (2007) In vivo response to vascular injury in the absence of factor IX: examination in factor IX knockout mice. Thromb Res 121:225-34
Davis, Charles H; Deerfield 2nd, David; Wymore, Troy et al. (2007) A quantum chemical study of the mechanism of action of Vitamin K carboxylase (VKC) III. Intermediates and transition states. J Mol Graph Model 26:409-14

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