Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL058141-05
Application #
6389663
Study Section
Hematology Subcommittee 2 (HEM)
Program Officer
Link, Rebecca P
Project Start
1997-04-01
Project End
2002-03-31
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
5
Fiscal Year
2001
Total Cost
$351,733
Indirect Cost
Name
Washington University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Vicente, Cristina P; Weiler, Hartmut; Di Cera, Enrico et al. (2012) Thrombomodulin is required for the antithrombotic activity of thrombin mutant W215A/E217A in a mouse model of arterial thrombosis. Thromb Res 130:646-8
Niu, Weiling; Chen, Zhiwei; Gandhi, Prafull S et al. (2011) Crystallographic and kinetic evidence of allostery in a trypsin-like protease. Biochemistry 50:6301-7
Gohara, David W; Di Cera, Enrico (2011) Allostery in trypsin-like proteases suggests new therapeutic strategies. Trends Biotechnol 29:577-85
Pozzi, Nicola; Chen, Raymond; Chen, Zhiwei et al. (2011) Rigidification of the autolysis loop enhances Na(+) binding to thrombin. Biophys Chem 159:6-13
Di Cera, Enrico (2011) Thrombin as an anticoagulant. Prog Mol Biol Transl Sci 99:145-84
Rana, Sadhna; Pozzi, Nicola; Pelc, Leslie A et al. (2011) Redesigning allosteric activation in an enzyme. Proc Natl Acad Sci U S A 108:5221-5
Pozzi, Nicola; Chen, Zhiwei; Zapata, Fatima et al. (2011) Crystal structures of prethrombin-2 reveal alternative conformations under identical solution conditions and the mechanism of zymogen activation. Biochemistry 50:10195-202
Berny-Lang, Michelle A; Hurst, Sawan; Tucker, Erik I et al. (2011) Thrombin mutant W215A/E217A treatment improves neurological outcome and reduces cerebral infarct size in a mouse model of ischemic stroke. Stroke 42:1736-41
Gandhi, Prafull S; Chen, Zhiwei; Appelbaum, Eric et al. (2011) Structural basis of thrombin-protease-activated receptor interactions. IUBMB Life 63:375-82
Flick, Matthew J; Chauhan, Anil K; Frederick, Malinda et al. (2011) The development of inflammatory joint disease is attenuated in mice expressing the anticoagulant prothrombin mutant W215A/E217A. Blood 117:6326-37

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