Ethanol use can be an important source of morbidity and mortality worldwide. It is known that half of individuals with significant burn injuries necessitating hospitalization were positive for ethanol (EtOH). Statistically speaking, the majority of these individuals were occasional binge drinkers, not alcoholics. Ethanol use at the time of injury also results in increased morbidity and mortality (> 2-fold) in the burned patients. Validated murine models combining EtOH + thermal burn injury have demonstrated massive acute systemic cytokine release with toxicity of these combined agents involving lung, small intestine and liver, which mimics human pathology. The process by which EtOH augments thermal burn injury-mediated toxicity has been unknown, yet, we have demonstrated a novel mechanism involving the lipid mediator Platelet-activating Factor (1-alkyl-2-acetyl glycerophosphocholine; PAF). This proposal is the renewal of a long-standing grant that has served to characterize PAF effects, focusing on skin pathophysiology. Exerting its effects via a specific G-protein coupled receptor (PAFR) widely expressed, acute PAF exposure results in a systemic pro-inflammatory response that mimics septic shock. Our ongoing and published studies accomplished in the last cycle of this grant have discovered that acute exposure of keratinocytes in vitro or mice in vivo with EtOH followed by thermal burn injury results in a tremendous increase in PAF production, mediated by augmented cytosolic phospholipase A2 (cPLA2) activation. Of importance, PAFR KO mice were completely protected from intoxicated thermal burn injury- induced multi-organ inflammation. Moreover, we discovered that PAF leaves the keratinocyte via subcellular microvesicle particles (MVP) in a process dependent upon PAFR activation and the enzyme acid sphingomyelinase (aSMase). We hypothesize that traveling in MVP protects PAF from degradation from PAF- acetyl hydrolases, and provides the lipid ligand to the PAFR in optimal form. The overall objective of the planned studies in this renewal is to address knowledge gaps in how PAF-filled MVPs are produced and exactly how they generate widespread multi-organ systemic inflammation in response to combining EtOH + thermal burn injury.
Three aims are designed to test the hypothesis that acute EtOH exposure bypasses the tightly regulated PAF synthetic and degradation pathways resulting in exaggerated PAF production in response to thermal burn injury, and that PAFR activation generates PAF-filled MVP via aSMase. The PAF-filled Burn-MVP travel systemically to induce gut bacterial translocation resulting in ?septic shock?.
The first aim will define the mechanisms for EtOH- mediated augmentation of MVP and PAF production.
The second aim will define if MVP serve to protect PAF from degradation and increases PAF's potency by providing the ligand embedded in membrane.
The third aim will define the exact mechanisms by which PAF in Burn-MVP generate systemic toxicity.
This aim will build upon our mechanistic studies to test potential treatment strategies for intoxicated thermal burn injury. These studies will address knowledge gaps and generate new therapeutic approaches for environmental injuries.
Short-term ethanol exposure combined with thermal burn injuries results in an acute systemic multi-organ dysfunction as well as delayed immunosuppression. The experiments described in this proposal are designed to test a novel hypothesis that short-term ethanol exposure results in an augmentation of the production of the bioactive lipid mediator Platelet-activating Factor following environmental skin injuries such as thermal burns or ultraviolet B radiation, which can explain the pathological acute and delayed effects of these combined stressors. The information derived from these studies will result in improved understanding and novel therapeutics for patients suffering environmental skin injuries in the setting of ethanol exposure.
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