The long-term goal of this proposal is to identify functions and determine mechanisms of the fibrinolytic system, and its inhibitors, in physiological and pathological processes utilizing cell-based and in vivo models. The availability of mice with deficiencies of genes of the fibrinolytic system has resulted in direct analyses of the role of these proteins in a number of biological events. Studies have indicated that a PAI-1 deficiency diminishes angiogenesis in tumor models. Further, our laboratory has shown that endothelial cell (EC) signaling and function are regulated by PAI-1/LRP interactions. The current application will further elucidate effects of PAI-1 on cell signaling pathways and determine the importance of PAI-1/LRP interactions in both cellular and physiological events. As a result of these observations, the following studies are proposed: (1.) Determine the effects of a PAI-1 deficiency on murine EC JAK/STAT signaling and cell cycle progression. These studies will assess STAT and JAK expression profiles and activation status in proliferating wild-type (WT) and PAI-1-/- EC as well as the extent of nuclear translocation of STAT. The addition of rPAI-1 and mutants will determine which functional domains of PAI-1 regulate the activation status of this pathway. Additional studies will determine effects on cell migration. Downstream effects on cell cycle progression will also be investigated. The hypothesis is that a PAI-1 deficiency will affect JAK/STAT signaling and downstream cell cycle progression, and that these effects are mediated by PAI-1/LRP interactions. (2.) Characterize early and late stage events of cardiac fibrosis in PAI-1-/- and uPA-/-/PAI-1-/- mice. Recent studies have shown that PAI-1-/- mice develop cardiac fibrosis, which may be mediated by dysregulated uPA or chronic activation of the Akt pathway, the result of altered PAI-1/LRP interactions. The studies proposed will initially characterize cardiac fibrosis in PAI-1-/- and uPA-/-/PAI-1-/- mice in order to differentiate effects from uPA activity and PAI-1 functions independent of uPA inhibition in cardiac fibrosis phenotypes. The hypothesis is that cardiac fibrosis will be regulated by urokinase activity and other functions of PAI-I which will be further pursued in future studies of mice expressing functional mutations of PAI-1.
Plasminogen activator inhibitor-1 (PAI-1) is a multifunctional protein that can affect cell function through altering cell adhesion to extracellular matrix by interaction with vitronectin (VN) and/or regulating internalization of urokinase (uPA)/urokinase receptor (uPAR) through interaction with low density lipoprotein receptor related protein (LRP). Our laboratory has determined that cell signaling and resultant effects on cell proliferation are regulated by PAI-1/LRP interactions. Results from these studies will facilitate an understanding of the effects of PAI-1 on endothelial cell function and biology and serve to identify its mechanistic involvement in the angiogenic process.
|Gupta, Kamlesh K; Xu, Zhi; Castellino, Francis J et al. (2016) Plasminogen activator inhibitor-1 stimulates macrophage activation through Toll-like Receptor-4. Biochem Biophys Res Commun 477:503-8|
|Narasaki, R; Xu, Z; Liang, Z et al. (2012) The vitronectin-binding domain of plasminogen activator inhibitor-1 plays an important functional role in lipopolysaccharide-induced lethality in mice. J Thromb Haemost 10:2618-21|
|Ploplis, Victoria A (2011) Effects of altered plasminogen activator inhibitor-1 expression on cardiovascular disease. Curr Drug Targets 12:1782-9|
|Xu, Zhi; Castellino, Francis J; Ploplis, Victoria A (2010) Plasminogen activator inhibitor-1 (PAI-1) is cardioprotective in mice by maintaining microvascular integrity and cardiac architecture. Blood 115:2038-47|
|Balsara, Rashna D; Ploplis, Victoria A (2008) Plasminogen activator inhibitor-1: the double-edged sword in apoptosis. Thromb Haemost 100:1029-36|
|Balsara, R D; Xu, Z; Ploplis, V A (2007) Targeting plasminogen activator inhibitor-1: role in cell signaling and the biology of domain-specific knock-in mice. Curr Drug Targets 8:982-95|
|Xu, Zhi; Balsara, Rashna D; Gorlatova, Natalia V et al. (2004) Conservation of critical functional domains in murine plasminogen activator inhibitor-1. J Biol Chem 279:17914-20|
|Busuttil, S J; Ploplis, V A; Castellino, F J et al. (2004) A central role for plasminogen in the inflammatory response to biomaterials. J Thromb Haemost 2:1798-805|
|Ploplis, Victoria A; Balsara, Rashna; Sandoval-Cooper, Mayra J et al. (2004) Enhanced in vitro proliferation of aortic endothelial cells from plasminogen activator inhibitor-1-deficient mice. J Biol Chem 279:6143-51|
|Sato, Jun; Schorey, Jeffrey; Ploplis, Victoria A et al. (2003) The fibrinolytic system in dissemination and matrix protein deposition during a mycobacterium infection. Am J Pathol 163:517-31|
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