Lymphangiosarcoma (LAS) is angiosarcoma with lymphatic differentiation that originates from the malignant transformation of endothelial cells (ECs). The etiology of LAS is largely unknown, although lymphatic malformation (e.g. chronic lymphedema in breast cancer patients) has been recognized as a risk factor for the disease. The long-term goal of the proposed studies is to understand the molecular and cellular mechanisms of lymphatic malformation and progression to LAS in order to develop new strategies for effective therapies of this deadly disease. In the previous funding period, we created a mouse model with inducible EC-specific deletion of Tsc1 which recapitulates salient features of human LAS, and showed that hyper-activation of mTORC1 and increased VEGF autocrine signaling in ECs were required for initiation and maintenance of LAS. In further preliminary studies, we discovered that maintenance of hyper-activation of mTORC1 in TSC-deficient cells required autophagy under glucose-starvation, but not amino acid-starvation or normal conditions. Moreover, autophagy maintains elevated levels of ATP specifically through lipid catabolism and generation of fatty acids as the main source of ATP under these energy stress conditions. In addition, consistent with clinical findings that malignant transformation of vascular malformation to angiosarcoma was accompanied with secondary mutations, preliminary studies employing whole exome sequencing (WES) of LAS samples from our Tsc1i?EC mice model identified secondary mutations in several genes including Cdk6, which is involved in cell cycle regulation and implicated in other cancers. Based on these strong preliminary studies and using our unique novel mouse models, we propose to 1) determine the mechanisms of autophagy in the regulation of lipolysis to produce fatty acids as an alternative fuel for ATP production to maintain mTORC1 hyper-activation in Tsc1-null vascular tumor cells; 2) examine the role of autophagy-mediated lipid catabolism in maintaining mTORC1 hyper-activation in lymphatic malformation and LAS in vivo and evaluate potential therapeutic efficacy of targeting autophagy-mediated lipolysis in mouse and PDX models of lymphatic malformation and LAS; and 3) explore the roles and mechanisms of secondary mutations in Cdk6 and other genes in the development of lymphatic malformation and progression to LAS. Together, the proposed studies to examine reverse regulation of mTORC1 by autophagy through novel mechanisms of lipid metabolism as well as secondary mutations that synergizing with mTORC1 signaling will provide significant mechanistic insights into the disease, which may contribute to novel therapies for this devastating disease.

Public Health Relevance

Our knowledge about the mechanisms underlying the development of lymphatic malformation and progression to LAS is very limited, and LAS remains as a deadly disease with no effective treatment at present. The characterization of key signaling pathways such as mTORC1 signaling and cellular processes like autophagy as well as cross-talk between them and lipid catabolism in the vascular tumor cells will significantly advance our fundamental knowledge about lymphatic malformation and LAS and contribute to novel therapies for the disease.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Project (R01)
Project #
Application #
Study Section
Tumor Cell Biology Study Section (TCB)
Program Officer
Schramm, Charlene A
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Cincinnati
Anatomy/Cell Biology
Schools of Medicine
United States
Zip Code
Chen, Song; Wang, Chenran; Yeo, Syn et al. (2016) Distinct roles of autophagy-dependent and -independent functions of FIP200 revealed by generation and analysis of a mutant knock-in mouse model. Genes Dev 30:856-69
Wang, Chenran; Chen, Song; Yeo, Syn et al. (2016) Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide. J Cell Biol 212:545-60
Sun, Shaogang; Chen, Song; Liu, Fei et al. (2015) Constitutive Activation of mTORC1 in Endothelial Cells Leads to the Development and Progression of Lymphangiosarcoma through VEGF Autocrine Signaling. Cancer Cell 28:758-772
Fang, Fang; Sun, Shaogang; Wang, Li et al. (2015) Neural Crest-Specific TSC1 Deletion in Mice Leads to Sclerotic Craniofacial Bone Lesion. J Bone Miner Res 30:1195-205
Chen, Xiao Lei; Nam, Ju-Ock; Jean, Christine et al. (2012) VEGF-induced vascular permeability is mediated by FAK. Dev Cell 22:146-57
Fan, Huaping; Guan, Jun-Lin (2011) Compensatory function of Pyk2 protein in the promotion of focal adhesion kinase (FAK)-null mammary cancer stem cell tumorigenicity and metastatic activity. J Biol Chem 286:18573-82
Peng, Xu; Guan, Jun-Lin (2011) Focal adhesion kinase: from in vitro studies to functional analyses in vivo. Curr Protein Pept Sci 12:52-67
Zhao, Xiaofeng; Guan, Jun-Lin (2011) Focal adhesion kinase and its signaling pathways in cell migration and angiogenesis. Adv Drug Deliv Rev 63:610-5
Li, Xiao-Yan; Zhou, Xiaoming; Rowe, R Grant et al. (2011) Snail1 controls epithelial-mesenchymal lineage commitment in focal adhesion kinase-null embryonic cells. J Cell Biol 195:729-38
Wang, Chenran; Yoo, Youngdong; Fan, Huaping et al. (2010) Regulation of Integrin ? 1 recycling to lipid rafts by Rab1a to promote cell migration. J Biol Chem 285:29398-405

Showing the most recent 10 out of 26 publications