This proposal will develop and apply new methods for differential proteomics. Our hypothesis is that 15N stable isotope labeling of rats will allow differential proteomics using mammals. The long term objective is to develop advanced proteomic capabilities that will: identify proteins in complex mixtures by shotgun proteomics, measure differences in abundance of protein species by quantitative differential proteomics, identify and quantify global differences in protein post-translational modifications, and identify sequence variations in complex protein mixtures. A centerpiece of these objectives is a method to metabolically label the proteins of rats with 15N for quantitative analysis of proteins and protein modifications. New methods will be used to prepare tissues from brain for analysis by shotgun proteomics. Cell membranes will be labeled and then digested to preserve protein topology information. Multidimensional separations will be advanced to increase the numbers of proteins that can be identified from stable isotope labeled peptide mixtures. These methods will be used to create differential proteomic profiles of cell membranes from the cerebellum, cerebrum, and striatum/hypothalamus of a fetal and an adolescent rat. Information from these studies will lead to the development of specific hypotheses of receptor and protein populations during brain development. These methods will enable the use of powerful genetic models of development and disease for whole organism proteomics. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
1R01MH067880-01
Application #
6602004
Study Section
Special Emphasis Panel (ZRG1-BECM (01))
Program Officer
Huerta, Michael F
Project Start
2003-04-01
Project End
2008-03-31
Budget Start
2003-04-01
Budget End
2004-03-31
Support Year
1
Fiscal Year
2003
Total Cost
$419,261
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Ma, Yuanhui; Yates 3rd, John R (2018) Proteomics and pulse azidohomoalanine labeling of newly synthesized proteins: what are the potential applications? Expert Rev Proteomics 15:545-554
Yates 3rd, John R (2018) Content Is King: Databases Preserve the Collective Information of Science. J Biomol Tech 29:1-3
Liu, Han-Hsuan; McClatchy, Daniel B; Schiapparelli, Lucio et al. (2018) Role of the visual experience-dependent nascent proteome in neuronal plasticity. Elife 7:
Beys-da-Silva, Walter O; Rosa, Rafael L; Santi, Lucélia et al. (2018) Zika Virus Infection of Human Mesenchymal Stem Cells Promotes Differential Expression of Proteins Linked to Several Neurological Diseases. Mol Neurobiol :
Hickox, Ann E; Wong, Ann C Y; Pak, Kwang et al. (2017) Global Analysis of Protein Expression of Inner Ear Hair Cells. J Neurosci 37:1320-1339
Lopez Aguilar, Aime; Gao, Yu; Hou, Xiaomeng et al. (2017) Profiling of Protein O-GlcNAcylation in Murine CD8+ Effector- and Memory-like T Cells. ACS Chem Biol 12:3031-3038
Ma, Yuanhui; McClatchy, Daniel B; Barkallah, Salim et al. (2017) HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification. J Proteome Res 16:2213-2220
Chu, Qian; Rathore, Annie; Diedrich, Jolene K et al. (2017) Identification of Microprotein-Protein Interactions via APEX Tagging. Biochemistry 56:3299-3306
Ganguly, Archan; Han, Xuemei; Das, Utpal et al. (2017) Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin. J Cell Biol 216:2059-2074
Chatterjee, Sandip; Stupp, Gregory S; Park, Sung Kyu Robin et al. (2016) A comprehensive and scalable database search system for metaproteomics. BMC Genomics 17:642

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