Activation of neurons in the basolateral amygdala (BLA) plays an essential role in the cellular processes that underlie the normal, adaptive, behavioral response to threatening, as well as rewarding, environmental stimuli. Importantly, release of the neurotransmitter dopamine has also been shown to play a central role in the response to threatening or rewarding stimuli. Moreover, several neuropsychiatric disorders such as schizophrenia, which are commonly associated with emotional disturbances, are thought to result, at least in part, from abnormal dopamine transmission. Compelling evidence now suggests that region-specific release of dopamine into the BLA is an absolute requirement for the formation of fearful memories. Hence, dopamine depletion prevents the formation of fear memories, an effect that can be rescued by allowing dopamine release to occur only within the BLA. More specifically, pharmacological agents that selectively modulate the activity of D1 family dopamine receptors (D1R, including D1 and D5) in the BLA can also modulate fear memory formation and consolidation. Significantly, gene knockout mice with a global deletion of D1 receptors show an impairment of fear memory formation. In other brain regions, D1 receptors are believed to activate the protein kinase-A (PKA) cascade. Importantly, inactivation of the PKA pathway impairs fear memory formation. Together, these data suggest that activation of the D1 - PKA cascade in the BLA may play a critical role in the formation of fear memories. Similarly, recent studies have indicated that synchronized neural activity, both within the BLA and between the BLA and target structures such as the medial prefrontal cortex (mPFC), play a major role in memory formation and recall. Dopamine has long been known to play a critical role in synchronizing neural activity. However, no study has systematically examined the molecular, cellular, and network-level mechanisms by which D1 receptor activation may facilitate fear memory formation. The studies outlined in this proposal are designed to address this significant knowledge gap. We have strong preliminary data to support our hypothesis that: D1 receptor activation in BLA principal neurons acts to facilitate synaptic plasticity by a PKA-dependent enhancement of intrinsic membrane oscillations and spike timing precision. The resulting highly synchronized firing of principal neurons in distinct frequency ranges, and subsequent phase locking of synchronized activity between the BLA and mPFC, facilitates fear memory formation.
The specific aims of this proposal have been designed to answer three specific questions relating to this hypothesis: SA#1. Does activation of the D1 - PKA cascade facilitate intrinsic oscillatory activity in the BLA? SA#2. Is activation of the D1 - PKA cascade necessary for synaptic plasticity in BLA afferent inputs? SA#3. Can activation of the D1 - PKA cascade facilitate coherent oscillations between the BLA and mPFC during fear learning?

Public Health Relevance

Compelling evidence suggests that dopamine release in the amygdala is a prerequisite for the formation and expression of fear memory, and long-term changes in dopaminergic signaling are thought to underlie a number of psychiatric disorders, including schizophrenia, that are often associated with disturbances of emotion. However, the cellular and subcellular mechanisms that underlie the dopamine effect on fear learning are poorly understood. This proposal will use electrophysiological, molecular-biological, and behavioral techniques to examine the effect of site-specific modulation of the dopamine system in the basolateral amygdala, with the goal of identifying novel points for clinical intervention in several psychiatric disorders.

National Institute of Health (NIH)
National Institute of Mental Health (NIMH)
Research Project (R01)
Project #
Application #
Study Section
Neurobiology of Motivated Behavior Study Section (NMB)
Program Officer
Nadler, Laurie S
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Emory University
Schools of Medicine
United States
Zip Code
Ryan, Steve; Li, Chenchen; Menigoz, Aurélie et al. (2018) Repeated shock stress facilitates basolateral amygdala synaptic plasticity through decreased cAMP-specific phosphodiesterase type IV (PDE4) expression. Brain Struct Funct 223:1731-1745
Hennessey, Thomas; Andari, Elissar; Rainnie, Donald G (2018) RDoC-based categorization of amygdala functions and its implications in autism. Neurosci Biobehav Rev 90:115-129
Guo, Ji-Dong; O'Flaherty, Brendan M; Rainnie, Donald G (2017) Serotonin gating of cortical and thalamic glutamate inputs onto principal neurons of the basolateral amygdala. Neuropharmacology 126:224-232
Ryan, Steven J; Ehrlich, David E; Rainnie, Donald G (2016) Morphology and dendritic maturation of developing principal neurons in the rat basolateral amygdala. Brain Struct Funct 221:839-54
Yaoyao Jia; Zheyuan Wang; Canales, Daniel et al. (2016) A wirelessly-powered homecage with animal behavior analysis and closed-loop power control. Conf Proc IEEE Eng Med Biol Soc 2016:6323-6326
Ehrlich, David E; Neigh, Gretchen N; Bourke, Chase H et al. (2015) Prenatal stress, regardless of concurrent escitalopram treatment, alters behavior and amygdala gene expression of adolescent female rats. Neuropharmacology 97:251-8
Ehrlich, David E; Rainnie, Donald G (2015) Prenatal Stress Alters the Development of Socioemotional Behavior and Amygdala Neuron Excitability in Rats. Neuropsychopharmacology 40:2135-45
Li, Chenchen; Rainnie, Donald G (2014) Bidirectional regulation of synaptic plasticity in the basolateral amygdala induced by the D1-like family of dopamine receptors and group II metabotropic glutamate receptors. J Physiol 592:4329-51
Hubert, G W; Li, C; Rainnie, D G et al. (2014) Effects of stress on AMPA receptor distribution and function in the basolateral amygdala. Brain Struct Funct 219:1169-79
Tooker, Angela; Madsen, Teresa E; Yorita, Allison et al. (2013) Microfabricated polymer-based neural interface for electrical stimulation/recording, drug delivery, and chemical sensing--development. Conf Proc IEEE Eng Med Biol Soc 2013:5159-62

Showing the most recent 10 out of 22 publications