Abstract

Synaptic vesicles are the characteristic morphological features of nerve terminals. They contain neurotransmitter which is release during electrical stimulation presumably by an exocytotic mechanism. Synaptic vesicles from the electric organ of Narcine brasiliensis contain antigens shared by no other major subcellular element. Antibodies to these antigens therefore allow vesicles to be recognized both biochemically and anatomically. Using both these procedures, antibody binding will be used to follow the fate of synaptic vesicle antigens during exocytosis and the membrane recycling events which follow exocytosis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS009878-15
Application #
3394076
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1978-09-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
15
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Jarousse, Nadine; Wilson, Joshua D; Arac, Demet et al. (2003) Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif. Traffic 4:468-78
Dasgupta, Shoumita; Kelly, Regis B (2003) Internalization signals in synaptotagmin VII utilizing two independent pathways are masked by intramolecular inhibitions. J Cell Sci 116:1327-37
Blumstein, J; Faundez, V; Nakatsu, F et al. (2001) The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes. J Neurosci 21:8034-42
de Wit, H; Lichtenstein, Y; Kelly, R B et al. (2001) Rab4 regulates formation of synaptic-like microvesicles from early endosomes in PC12 cells. Mol Biol Cell 12:3703-15
Jarousse, N; Kelly, R B (2001) The AP2 binding site of synaptotagmin 1 is not an internalization signal but a regulator of endocytosis. J Cell Biol 154:857-66
Jarousse, N; Kelly, R B (2001) Endocytotic mechanisms in synapses. Curr Opin Cell Biol 13:461-9
Qualmann, B; Kelly, R B (2000) Syndapin isoforms participate in receptor-mediated endocytosis and actin organization. J Cell Biol 148:1047-62
Faundez, V V; Kelly, R B (2000) The AP-3 complex required for endosomal synaptic vesicle biogenesis is associated with a casein kinase Ialpha-like isoform. Mol Biol Cell 11:2591-604
Qualmann, B; Kessels, M M; Kelly, R B (2000) Molecular links between endocytosis and the actin cytoskeleton. J Cell Biol 150:F111-6
Marullo, S; Faundez, V; Kelly, R B (1999) Beta 2-adrenergic receptor endocytic pathway is controlled by a saturable mechanism distinct from that of transferrin receptor. Receptors Channels 6:255-69

Showing the most recent 10 out of 43 publications