Abstract

We are trying to unravel the mechanisms which control the functional properties of spinal cord neurons. We have found that muscle cells grown in culture produce a high molecular weight substance which can enhance the growth of the axons of spinal cord neurons. In this coming year we plan to make more accurate measurements of the chemical properties of this trophic substance. We will use gel filtration and ultracentrifuge sedimentation techniques to obtain measures of its molecular weight. Isoelectric focusing and ion exchange chromotography will be used in determining its isoelectric point. Using these techniques together with affinity chromotagraphy methods we will continue to improve purification methods for this trophic molecule. We will also try to elucidate the mechanism of activation of the slow potassium conductance system found in motoneurons and muscle cells. This year we hope to determine how membrane depolarization and internal calcium contribute to the activation of this conductance system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS012207-11
Application #
3394755
Study Section
Physiology Study Section (PHY)
Project Start
1978-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
11
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Miami School of Medicine
Department
Type
Schools of Medicine
DUNS #
City
Miami
State
FL
Country
United States
Zip Code
33101

  Publications

White, Michael G; Saleh, Osama; Nonner, Doris et al. (2012) Mitochondrial dysfunction induced by heat stress in cultured rat CNS neurons. J Neurophysiol 108:2203-14
Chaverneff, Florence; Barrett, John (2009) Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress. J Neurochem 109:733-43
Panickar, Kiran S; Nonner, Doris; White, Michael G et al. (2008) Overexpression of Cdk5 or non-phosphorylatable retinoblastoma protein protects septal neurons from oxygen-glucose deprivation. Neurochem Res 33:1852-8
Talbot, Janet D; Barrett, John N; Barrett, Ellen F et al. (2008) Rapid, stimulation-induced reduction of C12-resorufin in motor nerve terminals: linkage to mitochondrial metabolism. J Neurochem 105:807-19
White, Michael G; Luca, Luminita E; Nonner, Doris et al. (2007) Cellular mechanisms of neuronal damage from hyperthermia. Prog Brain Res 162:347-71
Talbot, Janet; Barrett, John N; Barrett, Ellen F et al. (2007) Stimulation-induced changes in NADH fluorescence and mitochondrial membrane potential in lizard motor nerve terminals. J Physiol 579:783-98
Panickar, K S; Nonner, D; Barrett, J N (2005) Overexpression of Bcl-xl protects septal neurons from prolonged hypoglycemia and from acute ischemia-like stress. Neuroscience 135:73-80
White, Michael G; Emery, Michael; Nonner, Doris et al. (2003) Caspase activation contributes to delayed death of heat-stressed striatal neurons. J Neurochem 87:958-68
Vila, Lizette; Barrett, Ellen F; Barrett, John N (2003) Stimulation-induced mitochondrial [Ca2+] elevations in mouse motor terminals: comparison of wild-type with SOD1-G93A. J Physiol 549:719-28
Nonner, D; Barrett, E F; Barrett, J N (2000) Brief exposure to neurotrophins produces a calcium-dependent increase in choline acetyltransferase activity in cultured rat septal neurons. J Neurochem 74:988-99

Showing the most recent 10 out of 28 publications