Abstract

The specific aims of the proposed investigation are all concerned with trace measurements of monamines released from nerve terminals in the vertebrate brain, especially in the corpus striatum. They are: 1) An experimental evaluation of the electrochemical response of a flow-cell using a pyrolytic graphite electrode, and the determination of the optimum geometry for this electrode in push-pull perfusion applications. This electrode will be used as a continuous monitor of the concentration of electroactive species in the perfusates of rat-brain regions and will allow time resolution of better than one second. 2) The construction and electrochemical evaluation of microvoltammetric electrodes from carbon fibers. Multiple arrays of these electrodes will be implanted in discrete regions of the rat brain to measure changes in concentration of electroactive species. The results from part 1) of this section will be used to identify the substances detected by these small electrodes. 3) An investigation of the effects of GABA and acetylcholine on the release of dopamine and serotonin in the corpus striatum of the rat using electochemical methods of detection. Measurements will be made in synaptosomes, brain slices, and in vivo. A correlation of the experimental results will provide information concerning the relative importance of these modulators on the release of monoamines in this brain region.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS015841-09
Application #
3396494
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1980-01-01
Project End
1988-12-31
Budget Start
1988-01-01
Budget End
1988-12-31
Support Year
9
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Indiana University Bloomington
Department
Type
Schools of Arts and Sciences
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47402

  Publications

Dankoski, Elyse C; Carroll, Susan; Wightman, Robert Mark (2016) Acute selective serotonin reuptake inhibitors regulate the dorsal raphe nucleus causing amplification of terminal serotonin release. J Neurochem 136:1131-1141
Bucher, Elizabeth S; Wightman, R Mark (2015) Electrochemical Analysis of Neurotransmitters. Annu Rev Anal Chem (Palo Alto Calif) 8:239-61
Fox, Megan E; Studebaker, R Isaac; Swofford, Nathaniel J et al. (2015) Stress and Drug Dependence Differentially Modulate Norepinephrine Signaling in Animals with Varied HPA Axis Function. Neuropsychopharmacology 40:1752-61
Dengler, Adam K; Wightman, R Mark; McCarty, Gregory S (2015) Microfabricated Collector-Generator Electrode Sensor for Measuring Absolute pH and Oxygen Concentrations. Anal Chem 87:10556-64
Park, Jinwoo; Bucher, Elizabeth S; Budygin, Evgeny A et al. (2015) Norepinephrine and dopamine transmission in 2 limbic regions differentially respond to acute noxious stimulation. Pain 156:318-27
Dankoski, Elyse C; Agster, Kara L; Fox, Megan E et al. (2014) Facilitation of serotonin signaling by SSRIs is attenuated by social isolation. Neuropsychopharmacology 39:2928-37
Park, Jinwoo; Bucher, Elizabeth S; Fontillas, Khristy et al. (2013) Opposing catecholamine changes in the bed nucleus of the stria terminalis during intracranial self-stimulation and its extinction. Biol Psychiatry 74:69-76
McElligott, Zoé A; Fox, Megan E; Walsh, Paul L et al. (2013) Noradrenergic synaptic function in the bed nucleus of the stria terminalis varies in animal models of anxiety and addiction. Neuropsychopharmacology 38:1665-73
Herr, Natalie Rios; Wightman, Robert Mark (2013) Improved techniques for examining rapid dopamine signaling with iontophoresis. Front Biosci (Elite Ed) 5:249-57
Hashemi, Parastoo; Dankoski, Elyse C; Lama, Rinchen et al. (2012) Brain dopamine and serotonin differ in regulation and its consequences. Proc Natl Acad Sci U S A 109:11510-5

Showing the most recent 10 out of 93 publications