This project will provide information about timing of RNA and protein syntheses necessary for the expression of specific neuronal phenotypes during development. We have described the sequence of appearance of some cellular properties of amphibian spinal neurons differentiating in vivo. The same developmental changes occur in embryonic spinal neurons dissociated and grown in culture. Specific metabolic inhibitors will be added to the culture media at later and later stages of development, and the time of application at which their continuous presence no longer blocks expression of electrical excitability, chemosensitivity and high affinity neurotransmitter uptake will be determined. Brief applications of reversible metabolic inhibitors will provide information about the existence of critical periods, during which expression of single phenotypes may be blocked. If such critical periods exist, it will be possible to examine the consequences of the absence of one phenotype on the expression of others.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS015918-09
Application #
3396560
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1980-01-01
Project End
1992-12-31
Budget Start
1988-01-01
Budget End
1988-12-31
Support Year
9
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
Organized Research Units
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Dulcis, Davide; Lippi, Giordano; Stark, Christiana J et al. (2017) Neurotransmitter Switching Regulated by miRNAs Controls Changes in Social Preference. Neuron 95:1319-1333.e5
Spitzer, Nicholas C (2015) Neurotransmitter Switching? No Surprise. Neuron 86:1131-44
Guemez-Gamboa, Alicia; Xu, Lin; Meng, Da et al. (2014) Non-cell-autonomous mechanism of activity-dependent neurotransmitter switching. Neuron 82:1004-16
Spitzer, Nicholas C; Borodinsky, Laura N; Root, Cory M (2013) Imaging and manipulating calcium transients in developing Xenopus spinal neurons. Cold Spring Harb Protoc 2013:653-64
Demarque, Michael; Spitzer, Nicholas C (2012) Neurotransmitter phenotype plasticity: an unexpected mechanism in the toolbox of network activity homeostasis. Dev Neurobiol 72:22-32
Dulcis, Davide; Spitzer, Nicholas C (2012) Reserve pool neuron transmitter respecification: Novel neuroplasticity. Dev Neurobiol 72:465-74
Spitzer, Nicholas C (2012) Activity-dependent neurotransmitter respecification. Nat Rev Neurosci 13:94-106
Rosenberg, Sheila S; Spitzer, Nicholas C (2011) Calcium signaling in neuronal development. Cold Spring Harb Perspect Biol 3:a004259
Nicol, Xavier; Hong, Kwan Pyo; Spitzer, Nicholas C (2011) Spatial and temporal second messenger codes for growth cone turning. Proc Natl Acad Sci U S A 108:13776-81
Velazquez-Ulloa, Norma A; Spitzer, Nicholas C; Dulcis, Davide (2011) Contexts for dopamine specification by calcium spike activity in the CNS. J Neurosci 31:78-88

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