Abstract

Previous research has identified a complex pattern of sensory modulation by cortical pathways. An important area of modulation is the dorsal column nuclei. Neurons in the dorsal column nuclei may receive input from either glutamergic or aspartergic cortical synapses. This input may occur on either interneurons or relay neurons. One area of the proposed research is to determine at the EM level, using multiple label techniques, the neurochemicals contained in afferents to identified subpopulations of dorsal column nuclei neurons. This initial work will be followed by identification of amino acid receptors that are expressed by dorsal column nuclei neurons. Immunocytochemical localization will be used, as well as in situ hybridization using oligonucleotide probes. Postembedding immunogold techniques are hoped to be developed to localize receptors at the EM level. Lastly, the proposal plans to continue analysis of NO neurons and confirm that all are indeed interneurons rather than neurons with other target areas. Again, using double label methodology, colocalizaton of NO with glycine rather than GABA in dorsal column nuclei neurons will be explored.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS016264-14A1
Application #
2262969
Study Section
Neurology B Subcommittee 2 (NEUB)
Project Start
1980-04-01
Project End
1996-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
14
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Boukhelifa, M; Parast, M M; Valtschanoff, J G et al. (2001) A role for the cytoskeleton-associated protein palladin in neurite outgrowth. Mol Biol Cell 12:2721-9
Popratiloff, A; Valtschanoff, J G; Rustioni, A et al. (1996) Colocalization of GABA and glycine in the rat dorsal column nuclei. Brain Res 706:308-12
De Biasi, S; Vitellaro-Zuccarello, L; Bernardi, P et al. (1995) Ultrastructural and immunocytochemical characterization of terminals of postsynaptic ascending dorsal column fibers in the rat cuneate nucleus. J Comp Neurol 353:109-18
Valtschanoff, J G; Weinberg, R J; Rustioni, A et al. (1995) Colocalization of neuronal nitric oxide synthase with GABA in rat cuneate nucleus. J Neurocytol 24:237-45
De Biasi, S; Vitellaro-Zuccarello, L; Bernardi, P et al. (1994) Ultrastructural and immunocytochemical characterization of primary afferent terminals in the rat cuneate nucleus. J Comp Neurol 347:275-87
Valtschanoff, J G; Weinberg, R J; Kharazia, V N et al. (1993) Neurons in rat cerebral cortex that synthesize nitric oxide: NADPH diaphorase histochemistry, NOS immunocytochemistry, and colocalization with GABA. Neurosci Lett 157:157-61
Valtschanoff, J G; Weinberg, R J; Kharazia, V N et al. (1993) Neurons in rat hippocampus that synthesize nitric oxide. J Comp Neurol 331:111-21
Battaglia, G; Rustioni, A (1992) Substance P innervation of the rat and cat thalamus. II. Cells of origin in the spinal cord. J Comp Neurol 315:473-86
Battaglia, G; Spreafico, R; Rustioni, A (1992) Substance P innervation of the rat and cat thalamus. I. Distribution and relation to ascending spinal pathways. J Comp Neurol 315:457-72
Phend, K D; Weinberg, R J; Rustioni, A (1992) Techniques to optimize post-embedding single and double staining for amino acid neurotransmitters. J Histochem Cytochem 40:1011-20

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