This application is for the continuation of the investigator's research program on the """"""""cell body response"""""""" to axotomy. The proposed research will be performed using neurons and non-neuronal cells derived from the adult superior ganglion in both in situ and in vitro studies. The grant will test a provocative model that attempts to explain the changes in gene expression following neuronal injury. The experiments to test the model involve in situ hybridization and immunohistochemistry of the SCG neurons and non-neuronal cells. In addition, biochemical experiments are proposed to purify a LIF-inducing factor using this preparation. Explanted cultures of adult ganglia will also be examined for changes in both gene and peptide expression using primary culture techniques and molecular techniques, including northern blot and western blot, using phosphospecific antibodies to target transcription factors.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS017512-16
Application #
2858098
Study Section
Neurology B Subcommittee 2 (NEUB)
Program Officer
Michel, Mary E
Project Start
1981-07-01
Project End
2001-12-31
Budget Start
1999-01-01
Budget End
1999-12-31
Support Year
16
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Neurosciences
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Niemi, Jon P; Filous, Angela R; DeFrancesco, Alicia et al. (2017) Injury-induced gp130 cytokine signaling in peripheral ganglia is reduced in diabetes mellitus. Exp Neurol 296:1-15
Lingappa, Jaisri R; Zigmond, Richard E (2013) Limited recovery of pineal function after regeneration of preganglionic sympathetic axons: evidence for loss of ganglionic synaptic specificity. J Neurosci 33:4867-74
Niemi, Jon P; DeFrancesco-Lisowitz, Alicia; Roldán-Hernández, Lilinete et al. (2013) A critical role for macrophages near axotomized neuronal cell bodies in stimulating nerve regeneration. J Neurosci 33:16236-48
Zigmond, Richard E (2012) Cytokines that promote nerve regeneration. Exp Neurol 238:101-6
Pellegrino, Michael J; Parrish, Diana C; Zigmond, Richard E et al. (2011) Cytokines inhibit norepinephrine transporter expression by decreasing Hand2. Mol Cell Neurosci 46:671-80
Hyatt Sachs, H; Rohrer, H; Zigmond, R E (2010) The conditioning lesion effect on sympathetic neurite outgrowth is dependent on gp130 cytokines. Exp Neurol 223:516-22
Habecker, Beth A; Sachs, Hilary Hyatt; Rohrer, Hermann et al. (2009) The dependence on gp130 cytokines of axotomy induced neuropeptide expression in adult sympathetic neurons. Dev Neurobiol 69:392-400
Sachs, Hilary H; Wynick, David; Zigmond, Richard E (2007) Galanin plays a role in the conditioning lesion effect in sensory neurons. Neuroreport 18:1729-33
Hyatt Sachs, H; Schreiber, R C; Shoemaker, S E et al. (2007) Activating transcription factor 3 induction in sympathetic neurons after axotomy: response to decreased neurotrophin availability. Neuroscience 150:887-97
Zigmond, Richard E; Vaccariello, Stacey A (2007) Activating transcription factor 3 immunoreactivity identifies small populations of axotomized neurons in rat cervical sympathetic ganglia after transection of the preganglionic cervical sympathetic trunk. Brain Res 1159:119-23

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