The paraventricular nucleus (PVN) of hypothalamus is emerging as a central site for the integration and coordination of some neuroendocrine and autonomic visceral responses and its importance in the maintenance of homoeostasis is proposed. Efferent fiber projections of PV neurons to the pituitary gland and specific brainstem centers associated with the control of cardiovascular and respiratory functions are known. The studies proposed will further our understanding of the organization of afferent peptidergic fiber projections to the paraventricular nucleus and elucidate the significance of these pathways in relation to the neuroendocrine and autonomic functions of the PVN.
The specific aims of this proposal are (1) to describe the cytoarchitectonic organization of ACTH/Beta-endorphin, enkephalin and somatostatin immunoreactive neurons within the functionally distinct subnuclei of the PVN complex and (2) to elucidate the instrinsic synaptic organization and potential interactions and communications of these three central peptidergic systems and magnocellular (vasopressin/oxytocin) PV neurons. The studies proposed will provide an anatomical substrate for functional interactions described between each of the selected neuropeptidergic systems and magnocellular neurons to occur. These findings will be of considerable importance to the neuroendocrinologist interested in the control mechanism of vasopressin and oxytocin secretion and their effects on neuroendocrine and autonomic visceral (e.g. cardiovascular) functions. Anatomical details will be elucidated using Vibratome tissue sections and the basic and double antigen immunocytochemical procedures at both the light and electron microscopic levels.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Project (R01)
Project #
Application #
Study Section
Neurology A Study Section (NEUA)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Rochester
Schools of Dentistry
United States
Zip Code
Piekut, D T; Phipps, B (1999) Corticotropin-releasing factor--immunolabeled fibers in brain regions with localized kainate neurotoxicity. Acta Neuropathol (Berl) 98:622-8
Kellogg, C K; Awatramani, G B; Piekut, D T (1998) Adolescent development alters stressor-induced Fos immunoreactivity in rat brain. Neuroscience 83:681-9
Piekut, D T; Phipps, B (1998) Increased corticotropin-releasing factor immunoreactivity in select brain sites following kainate elicited seizures. Brain Res 781:100-13
Callahan, T A; Piekut, D T (1997) Differential Fos expression induced by IL-1beta and IL-6 in rat hypothalamus and pituitary gland. J Neuroimmunol 73:207-11
Pretel, S; Applegate, C D; Piekut, D T (1996) The kindling-activated neuronal network: recruitment of somatostatin-synthesizing neurons. Brain Res Bull 41:237-47
Sun, Q; Pretel, S; Applegate, C D et al. (1996) Oxytocin and vasopressin mRNA expression in rat hypothalamus following kainic acid-induced seizures. Neuroscience 71:543-54
Piekut, D T; Pretel, S; Applegate, C D (1996) Activation of oxytocin-containing neurons of the paraventricular nucleus (PVN) following generalized seizures. Synapse 23:312-20
Piekut, D; Phipps, B; Pretel, S et al. (1996) Effects of generalized convulsive seizures on corticotropin-releasing factor neuronal systems. Brain Res 743:63-9
Piekut, D T; Phipps, B (1996) Corticotropin-releasing factor induction in rat piriform cortex following kainate-elicited seizures. Neurosci Lett 209:45-8
Pretel, S; Applegate, C D; Piekut, D T (1995) Seizure-induced activation of enkephalin- and somatostatin-synthesizing neurons. Peptides 16:951-7

Showing the most recent 10 out of 32 publications