Abstract

Nerve growth factor (NGF) is a protein required for the development, maintenance, and repair of the nervous system. There are two NGF receptors, gp75 and the tyrosine kinase TrkA. Both of these receptors can signal separately and they synergize with each other to activate multiple signal transduction pathways. The investigators hypothesize that NGF-induced receptor dimerization and clustering are the initiating These studies may provide a physical basis for NGF receptor signaling and, thereby, aid the development of new approaches for enhancing neuronal survival following trauma or during neurodegenerative disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
3R01NS028760-08S1
Application #
6319185
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Leblanc, Gabrielle G
Project Start
1993-08-01
Project End
2002-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
8
Fiscal Year
2000
Total Cost
$50,000
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Physiology
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655

  Publications

Bulseco, Dylan A; Wolf, David E (2013) Fluorescence correlation spectroscopy: molecular complexing in solution and in living cells. Methods Cell Biol 114:489-524
Bulseco, Dylan A; Wolf, David E (2007) Fluorescence correlation spectroscopy: molecular complexing in solution and in living cells. Methods Cell Biol 81:525-59
Bulseco, Dylan A; Wolf, David E (2003) Fluorescence correlation spectroscopy: molecular complexing in solution and in living cells. Methods Cell Biol 72:465-98
Wolf, David E (2003) Quantitative digital and video microscopy. Methods Cell Biol 72:319-36
Wolf, David E (2003) Fundamentals of fluorescence and fluorescence microscopy. Methods Cell Biol 72:157-84
Wolf, David E (2003) The optics of microscope image formation. Methods Cell Biol 72:11-43
Bulseco, D A; Poluha, W; Schonhoff, C M et al. (2001) Cell-cycle arrest in TrkA-expressing NIH3T3 cells involves nitric oxide synthase. J Cell Biochem 81:193-204
Ross, A H; Lachyankar, M B; Recht, L D (2001) PTEN: a newly identified regulator of neuronal differentiation. Neuroscientist 7:278-81
Schonhoff, C M; Bulseco, D A; Brancho, D M et al. (2001) The Ras-ERK pathway is required for the induction of neuronal nitric oxide synthase in differentiating PC12 cells. J Neurochem 78:631-9
Lachyankar, M B; Sultana, N; Schonhoff, C M et al. (2000) A role for nuclear PTEN in neuronal differentiation. J Neurosci 20:1404-13

Showing the most recent 10 out of 22 publications