Abstract

The investigator has found a sequence element in the APP promoter that is essential for high levels of expression. The sequence element contains a binding domain for a novel nuclear factor that activates transcription from the APP promoter. The investigator proposes to purify the factor by affinity chromatography, determine the subunit composition, clone the factor. The mechanism via which the factor operates will addressed by mutagenesis and in vitro transcription.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS030994-05
Application #
2037581
Study Section
Neurology C Study Section (NEUC)
Program Officer
Oliver, Eugene J
Project Start
1992-07-01
Project End
2001-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Psychiatry
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Quitschke, Wolfgang W (2008) Differential solubility of curcuminoids in serum and albumin solutions: implications for analytical and therapeutic applications. BMC Biotechnol 8:84
Pugacheva, Elena M; Tiwari, Vijay Kumar; Abdullaev, Ziedulla et al. (2005) Familial cases of point mutations in the XIST promoter reveal a correlation between CTCF binding and pre-emptive choices of X chromosome inactivation. Hum Mol Genet 14:953-65
Loukinov, Dmitri I; Pugacheva, Elena; Vatolin, Sergei et al. (2002) BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma. Proc Natl Acad Sci U S A 99:6806-11
Vostrov, Alexander A; Taheny, Michael J; Quitschke, Wolfgang W (2002) A region to the N-terminal side of the CTCF zinc finger domain is essential for activating transcription from the amyloid precursor protein promoter. J Biol Chem 277:1619-27
Quitschke, W W; Taheny, M J; Fochtmann, L J et al. (2000) Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein gene. Nucleic Acids Res 28:3370-8
Vostrov, A A; Quitschke, W W (2000) Plasma hyaluronan-binding protein is a serine protease. J Biol Chem 275:22978-85
Yang, Y; Quitschke, W W; Vostrov, A A et al. (1999) CTCF is essential for up-regulating expression from the amyloid precursor protein promoter during differentiation of primary hippocampal neurons. J Neurochem 73:2286-98
Vostrov, A A; Quitschke, W W (1997) The zinc finger protein CTCF binds to the APBbeta domain of the amyloid beta-protein precursor promoter. Evidence for a role in transcriptional activation. J Biol Chem 272:33353-9
Quitschke, W W; Matthews, J P; Kraus, R J et al. (1996) The initiator element and proximal upstream sequences affect transcriptional activity and start site selection in the amyloid beta-protein precursor promoter. J Biol Chem 271:22231-9
Vostrov, A A; Quitschke, W W; Vidal, F et al. (1995) USF binds to the APB alpha sequence in the promoter of the amyloid beta-protein precursor gene. Nucleic Acids Res 23:2734-41

Showing the most recent 10 out of 12 publications