Abstract

Neurotrophins are known to control not only neuronal survival, but may modulate phenotypic differentiation and adult neuropathic pain. In transgenic mice overexpressing NT-3 or NGF, changes are noted in sensory neuron number, projections and pain threshold. The collaborating lab has the ability to determine the """"""""Comprehensive phenotype"""""""" of adult sensory neurons, using intracellular recording and labeling. These techniques will be used to examine individual sensory neurons of control and transgenic mice to assess if neurotrophin overexpression alters the phenotype (aims 1 and 2). It is also hypothesized that neuropathic pain recapitulates neurotrophin dependent processes.
In aim 3, the phenotype of neurons responsible for neuropathic pain will be characterized, and antineurotrophin antisera will be used to block formation of the sympathetic/sensory neuron connections thought to mediate neuropathic pain.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS031826-06
Application #
2669021
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Program Officer
Leblanc, Gabrielle G
Project Start
1993-05-01
Project End
2001-02-28
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Kentucky
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Smith-Edwards, Kristen M; DeBerry, Jennifer J; Saloman, Jami L et al. (2016) Profound alteration in cutaneous primary afferent activity produced by inflammatory mediators. Elife 5:
DeBerry, Jennifer J; Schwartz, Erica S; Davis, Brian M (2014) TRPA1 mediates bladder hyperalgesia in a mouse model of cystitis. Pain 155:1280-7
Wang, Ting; Jing, Xiaotang; DeBerry, Jennifer J et al. (2013) Neurturin overexpression in skin enhances expression of TRPM8 in cutaneous sensory neurons and leads to behavioral sensitivity to cool and menthol. J Neurosci 33:2060-70
Wang, Ting; Molliver, Derek C; Jing, Xiaotang et al. (2011) Phenotypic switching of nonpeptidergic cutaneous sensory neurons following peripheral nerve injury. PLoS One 6:e28908
Malin, Sacha; Molliver, Derek; Christianson, Julie A et al. (2011) TRPV1 and TRPA1 function and modulation are target tissue dependent. J Neurosci 31:10516-28
Koerber, H Richard; McIlwrath, Sabrina L; Lawson, Jeffrey J et al. (2010) Cutaneous C-polymodal fibers lacking TRPV1 are sensitized to heat following inflammation, but fail to drive heat hyperalgesia in the absence of TPV1 containing C-heat fibers. Mol Pain 6:58
Stucky, Cheryl L; Dubin, Adrienne E; Jeske, Nathaniel A et al. (2009) Roles of transient receptor potential channels in pain. Brain Res Rev 60:2-23
Malin, Sacha A; Christianson, Julie A; Bielefeldt, Klaus et al. (2009) TPRV1 expression defines functionally distinct pelvic colon afferents. J Neurosci 29:743-52
Malin, Sacha A; Davis, Brian M; Koerber, H Richard et al. (2008) Thermal nociception and TRPV1 function are attenuated in mice lacking the nucleotide receptor P2Y2. Pain 138:484-96
Elitt, Christopher M; Malin, Sacha A; Koerber, H Richard et al. (2008) Overexpression of artemin in the tongue increases expression of TRPV1 and TRPA1 in trigeminal afferents and causes oral sensitivity to capsaicin and mustard oil. Brain Res 1230:80-90

Showing the most recent 10 out of 41 publications