This application is focused on the following specific objectives: (1) development and characterization of further inbred lines of S. purpuratus, the species serving as the major sea urchin research model in the United States; (2) measurement, characterization, and collection of relevant naturally occurring zygotic recessives that are revealed in sibling inbred crosses, and that affect developmental processes (embryonic and larval); (3) development of methods and results of mutagenesis protocols exploiting the specific character of gametogenesis in sea urchins, and use of these protocols to search for maternally acting genetic functions; (4) development of permanent S. purpuratus tissue culture cell lines, utilizing T-Antigen transformed mesenchyme and other cells; (5) determination of the source of the germ line in sea urchins, using microsurgery and postembryonic lineage tracing by recombinational marker activation; (6) development of laboratory protocols for alterations of sex determination physiology in order to produce hermaphrodites suitable for research; and (7) development of a family of gene trap vectors for sea urchin embryos.
| Cameron, R Andrew; Rast, Jonathan P; Brown, C Titus (2004) Genomic resources for the study of sea urchin development. Methods Cell Biol 74:733-57 |
| Howard, Meredith L; Davidson, Eric H (2004) cis-Regulatory control circuits in development. Dev Biol 271:109-18 |
| Cameron, R Andrew; Oliveri, Paola; Wyllie, Jane et al. (2004) cis-Regulatory activity of randomly chosen genomic fragments from the sea urchin. Gene Expr Patterns 4:205-13 |
| Amore, Gabriele; Yavrouian, Robert G; Peterson, Kevin J et al. (2003) Spdeadringer, a sea urchin embryo gene required separately in skeletogenic and oral ectoderm gene regulatory networks. Dev Biol 261:55-81 |
| Davidson, Eric H; McClay, David R; Hood, Leroy (2003) Regulatory gene networks and the properties of the developmental process. Proc Natl Acad Sci U S A 100:1475-80 |
| Rast, Jonathan P (2003) Development gene networks and evolution. J Struct Funct Genomics 3:225-34 |
| Calestani, Cristina; Rast, Jonathan P; Davidson, Eric H (2003) Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening. Development 130:4587-96 |
| Davidson, Eric H; Rast, Jonathan P; Oliveri, Paola et al. (2002) A genomic regulatory network for development. Science 295:1669-78 |
| Ransick, Andrew; Rast, Jonathan P; Minokawa, Takuya et al. (2002) New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization. Dev Biol 246:132-47 |
| Rast, Jonathan P; Cameron, R Andrew; Poustka, Albert J et al. (2002) brachyury Target genes in the early sea urchin embryo isolated by differential macroarray screening. Dev Biol 246:191-208 |
Showing the most recent 10 out of 15 publications
