There are persistent questions regarding the evolution and function of the immune system. Previously studies performed in non-mammalian vertebrates have helped to reveal basic features of the immune system that are universal. In other cases, studies outside of mouse and human have underscored the great plasticity seen in certain genes/molecules/mechanisms related to immunity. In our studies of the immune system in cartilaginous fish (sharks, skates, and rays), the oldest animals with adaptive immunity, we have discovered a new immunoglobulin (Ig) isotype (the new antigen receptor or IgNAR) with a very unusual binding site composed of a so-called single-chain variable (V) region. This unusual antibody is of potential therapeutic/diagnostic interest, but for this proposal it has opened new avenues to examine antibody structure and function throughout evolution. Because of their atypical gene organization, we propose that the cartilaginous fish Ig genes are expressed by cells that are 'layered'in the immune system: 'innate'receptors with fixed specificities expressed first in development, followed by cells with rearranged IgV receptors expressed as multimeric IgM, followed by lymphocytes that produce monomeric IgM or IgNAR. We propose that the 'innate'receptors and 19S IgM form a first line of defense, and the 7S and IgNAR provide the highly specific humoral immunity. Unexpectedly, the NAR V domain is also used by a subset of ??? T cell receptors (TCR), and we propose that such receptors complement the canonical pool of ??? TCR, in the same ways that IgNAR is believed to complement IgM. Finally, an IgD homologue was discovered that was revealed to be an orthologue of a close cousin of IgNAR, IgW. The function of IgD in humans is not known, and we propose that an understanding its ancestry could be useful in unraveling its role in generating immune responses. Public information: Unusual animal models found at crucial steps in the evolutionary tree are used to help understand basic features of the human immune system-we use sharks, the oldest living animals with an adaptive immune system, and Xenopus, an amphibian at the transition point between aquatic and terrestrial vertebrates. We are especially interested in studying the structure and function of human immune molecules (the antibody isotype IgD and the ??? T cell receptor), whose functions have not been well defined.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Project (R01)
Project #
5R01RR006603-20
Application #
8039998
Study Section
Special Emphasis Panel (ZRG1-IMM-C (02))
Program Officer
Moro, Manuel H
Project Start
1991-02-11
Project End
2013-02-28
Budget Start
2011-03-01
Budget End
2013-02-28
Support Year
20
Fiscal Year
2011
Total Cost
$319,653
Indirect Cost
Name
University of Maryland Baltimore
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Redmond, Anthony K; Ohta, Yuko; Criscitiello, Michael F et al. (2018) Haptoglobin Is a Divergent MASP Family Member That Neofunctionalized To Recycle Hemoglobin via CD163 in Mammals. J Immunol 201:2483-2491
Neely, Harold R; Flajnik, Martin F (2015) CXCL13 responsiveness but not CXCR5 expression by late transitional B cells initiates splenic white pulp formation. J Immunol 194:2616-23
Venkatesh, Byrappa; Lee, Alison P; Ravi, Vydianathan et al. (2014) Elephant shark genome provides unique insights into gnathostome evolution. Nature 505:174-9
Castro, Caitlin D; Ohta, Yuko; Dooley, Helen et al. (2013) Noncoordinate expression of J-chain and Blimp-1 define nurse shark plasma cell populations during ontogeny. Eur J Immunol 43:3061-75
Smith, Lauren E; Crouch, Kathryn; Cao, Wei et al. (2012) Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias). Dev Comp Immunol 36:665-79
Flajnik, Martin F; Tlapakova, Tereza; Criscitiello, Michael F et al. (2012) Evolution of the B7 family: co-evolution of B7H6 and NKp30, identification of a new B7 family member, B7H7, and of B7's historical relationship with the MHC. Immunogenetics 64:571-90
Criscitiello, Michael F; Ohta, Yuko; Saltis, Mark et al. (2010) Evolutionarily conserved TCR binding sites, identification of T cells in primary lymphoid tissues, and surprising trans-rearrangements in nurse shark. J Immunol 184:6950-60
Flajnik, Martin F (2010) All GOD's creatures got dedicated mucosal immunity. Nat Immunol 11:777-9
Flajnik, Martin F; Kasahara, Masanori (2010) Origin and evolution of the adaptive immune system: genetic events and selective pressures. Nat Rev Genet 11:47-59
Dooley, Helen; Buckingham, E Bryan; Criscitiello, Michael F et al. (2010) Emergence of the acute-phase protein hemopexin in jawed vertebrates. Mol Immunol 48:147-52

Showing the most recent 10 out of 55 publications