A novel, relatively inexpensive and accurate flow cytometric technique, which identifies and quantitates the number of peripheral blood mononuclear cells (PBMC) infected with the human immunodeficiency virus (HIV), will be used to monitor the effect of antiviral therapies such as Zidovudine (AZT), dideoxycytidine (ddC), and dideoxyinosine (ddi) on the number of HIV- infected cells in AIDS patients undergoing these treatments. In addition, the effects of these antiviral compounds on the amount of free virus in the circulation will be measured using the p24 antigen-capture enzyme-linked immunosorbant assay (ELISA). We expect to be able to measure a decrease in the amount of HIV in the mononuclear cells using the flow cytometric technique and a decrease in the amount of free virus in the plasma using the p24 antigen-capture ELISA technique. These studies comparing the ability of these two techniques to measure the amount of virus in the circulation of AIDS patients undergoing chemotherapy for their disease should lead to useful and meaningful assays for monitoring the effect of antiviral therapies on the amount of cell-associated and cell-free HIV in these patients. Any decreases in the amount of HIV in the circulation will be correlated with the improvement in clinical status of the patient and other markers of improvement in these HIV-infected patients.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Research Grants (R03)
Project #
1R03AI030883-01
Application #
3422605
Study Section
AIDS and Related Research Study Section 4 (ARRD)
Project Start
1990-09-30
Project End
1991-08-31
Budget Start
1990-09-30
Budget End
1991-08-31
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Albany Medical College
Department
Type
Schools of Medicine
DUNS #
City
Albany
State
NY
Country
United States
Zip Code
12208
Remick, S C; Sedransk, N; Haase, R et al. (1999) Oral combination chemotherapy in the management of AIDS-related lymphoproliferative malignancies. Drugs 58 Suppl 3:99-107
McSharry, J M; Lurain, N S; Drusano, G L et al. (1998) Flow cytometric determination of ganciclovir susceptibilities of human cytomegalovirus clinical isolates. J Clin Microbiol 36:958-64
McSharry, J J (1994) Uses of flow cytometry in virology. Clin Microbiol Rev 7:576-604