Periodontal disease is a chronic inflammatory process in the periodontal tissue. It occurs in approximately 48% of the U.S. adult population and is the leading cause of tooth loss among adults. Guided tissue generation (GTR) is a widely-used, well-documented and clinically predictable technique to treat periodontal disease and dental/craniofacial defects by placing a polymeric membrane between epithelial tissue and periodontal ligament/bone tissue. This membrane serves as a barrier to protect the slower-growing periodontal ligament and bone tissues from the invasion of faster-growing epithelial tissue, but it should also allow exchange of fluids and signals between different tissues. A number of barrier membranes have been developed over the past decades, but current products are very costly and have limitations. Avian eggshell membrane is a semi-permeable fibrous membrane between egg-white and eggshell. It is available in abundant quantities as a waste product from the poultry and food industry. Natural eggshell membrane is composed of a highly-crosslinked interwoven protein fiber network with a unique double-layered structure. It not only allows excellent water/oxygen exchange and satisfies the need of embryo development, but also serves as a natural barrier membrane against the invasion of microorganisms and other challenges from the external environment. Inspired by the similarity of eggshell membrane and GTR membrane as a barrier membrane, we propose this project to explore the feasibility of developing an innovative and biomimetic artificial eggshell membrane for GTR application using a reactive electrospinning technique and response surface methodology (RSM), which can simulate the unique composition and microstructure of natural eggshell membrane. Our new membrane is a multiple-component system which contains soluble eggshell membrane protein (SEP), mechanically stable poly (lactic-co-glycolic acid) (PLGA) and osseoinductive nano-hydroxyapatite particles (nanoHAp). It also possesses a bio- inspired double-layered structure, which includes an outer barrier PLGA layer (adjacent to the gingival tissue) and an inner bioactive SEP/PLGA/nano-HAp layer (adjacent to the periodontal/bone tissue). The long-term goal of our proposed project is to improve the clinical outcome of the GTR technique by using this biomimetic membrane, as well as lay a foundation for converting eggshell membrane derived materials from an abundant industrial by-product to a new, green and promising biomaterial for dentistry and medicine.

Public Health Relevance

The guided tissue regeneration/guided bone regeneration (GTR/GBR) technique is a widely-used technique to treat periodontal disease and orofacial bone defects. A superior barrier membrane plays a key role for the successful application of GTR/GBR. The proposed project will develop an innovative artificial eggshell membrane for GTR/GBR applications, which can simulate the unique composition and microstructure of natural eggshell membrane by using the reactive electrospinning technique and response surface methodology (RSM).

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Small Research Grants (R03)
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NIDR Special Grants Review Committee (DSR)
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Lumelsky, Nadya L
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University of Mississippi Medical Center
Schools of Dentistry/Oral Hygn
United States
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