Stability is a critical problem for commercializing biotechnologically produced proteins of therapeutic values. Dr. Dong proposes to contribute to the solution of this problem through the investigation of protein folding/unfolding by means of FT-IR monitoring of the amide I band. He will initially investigate the stability of model proteins as a function of guanidine hydrochloride. He plans to eventually expand the study of the unfolding model system to proteins with diverse secondary structures and higher degrees of thermal and chemical stabilities.
| Dong, A; Randolph, T W; Carpenter, J F (2000) Entrapping intermediates of thermal aggregation in alpha-helical proteins with low concentration of guanidine hydrochloride. J Biol Chem 275:27689-93 |
| Dong, A; Meyer, J D; Brown, J L et al. (2000) Comparative fourier transform infrared and circular dichroism spectroscopic analysis of alpha1-proteinase inhibitor and ovalbumin in aqueous solution. Arch Biochem Biophys 383:148-55 |
