EDTA enhances the spermicidal activity of nonoxynol-9 (N9) in the presence of cervical mucus. Moreover, the combination of EDTA and N9 in cervical mucus appears to offer protection against sexually transmitted diseases including AIDS. To understand the fundamental basis of altered mucus receptivity to sperm, it is necessary to define the physical and biochemical changes associated with EDTA and N9 application to mucus. It is suggested the chelating action of EDTA alters the glycoprotein composition of cervical mucus. Correlations already exit between the levels of various mucin and protein components of mucus and viscoelasticity and sperm motility. It is suggested that EDTA may also affect such compositional changes physical properties and may also alter protein phosphorylation. In this proposal, the actions of EDTA on such parameters in cervical mucus will be examined.
|Cheeti, Sravanthi; Lee, Chi H (2010) The involvement of intracellular calcium in the MCT-mediated uptake of lactic acid by HeLa cells. Mol Pharm 7:169-76|
|Cheeti, Sravanthi; Warrier, Bharat K; Lee, Chi H (2006) The role of monocarboxylate transporters in uptake of lactic acid in HeLa cells. Int J Pharm 325:48-54|
|Wang, Yicheng; Lee, Chi H (2004) Effects of intrinsic variables on release of sodium dodecyl sulfate from a female controlled drug delivery system. Int J Pharm 282:173-81|
|Sang Choe, Eun; Warrier, Bharat; Soo Chun, Jang et al. (2004) EDTA-induced activation of Ca-regulated proteins in the vaginal mucosa. J Biomed Mater Res A 68:159-67|
|Warrier, Bharat K; Kostoryz, Elisabet; Lee, Chi H (2004) Biocompatibility of components of a female controlled drug delivery system. J Biomed Mater Res A 71:209-16|
|Lee, Chi H; Wang, Yicheng; Shin, Sang-Chul et al. (2002) Effects of chelating agents on the rheological property of cervical mucus. Contraception 65:435-40|
|Wang, Yicheng; Lee, Chi H (2002) Characterization of a female controlled drug delivery system for microbicides. Contraception 66:281-7|