Susceptibility determinants to Legionella pneumophila infection in smokers Abstract Legionella accounts for majority of drinking water-associated pneumonia outbreaks with high percentage of death. Legionella infection presents with a higher risk and worse outcome in the elderly, immune-compromised and smokers. Several epidemiological studies have demonstrated a strong association between tobacco smoking and an elevated risk of Legionella infection. However, the underlying mechanism is still unclear. To determine the mechanism by which smoking predisposes to Legionella infection, we will perform the following aims:
Aim 1. Determine the methylomic and RNAseq profile of human alveolar macrophages from current smokers (>10 pack years) and non-smokers.
Aim 2. Determine the function of genes with altered methylation and expression and identify those responsible for the increased susceptibility of macrophages to Legionella infection.

Public Health Relevance

Legionella tend to infect the elderly, people with low immunity and smokers causing pneumonia that is often fatal. In order to understand why smokers are prone to Legionella infection, we will obtain macrophages from the lungs of smokers and determine if they express genes different than those from non-smokers.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Exploratory/Developmental Grants (R21)
Project #
1R21AI156379-01
Application #
10106837
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Ernst, Nancy L
Project Start
2021-03-18
Project End
2023-02-28
Budget Start
2021-03-18
Budget End
2022-02-28
Support Year
1
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Ohio State University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
832127323
City
Columbus
State
OH
Country
United States
Zip Code
43210