Pasteurellosis is considered the leading cause of morbidity and mortality in rabbits and, as a consequence, is a significant complicating factor in biomedical research. The prevention of pasteurellosis in rabbits would remove a major impediment to the use of higher quality rabbits in research. Favorable protection against infection and disease caused by Pasteurella multocida has been reported in laboratory studies of Pasteurella-free rabbits using live streptomycin-dependent vaccines. Hence, such vaccines offer considerable promise in the control of pasteurellosis. A live streptomycin-dependent P. multocida vaccine will be evaluated, in the field, for safety and efficacy. Field evaluation of vaccine efficacy, in preventing infection and disease due to P. multocida, will be conducted at two commercial rabbitries. At one rabbitry, rabbits maintained at the rabbitry will be evaluated; white at the other rabbitry, the University's major supplier, rabbits will be evaluated before and after entry to the University's animal facility. An enzyme-linked immunosorbent assay will be used to measure immunoglobulin A and G antibodies in serum and nasal secretions. This should provide insight into the mechanism in vaccinated rabbits.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
5R24RR001326-02
Application #
3450521
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1984-09-01
Project End
1986-11-30
Budget Start
1985-09-01
Budget End
1986-11-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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Digiacomo, R F; Allen, V; Hinton, M H (1991) Naturally acquired Pasteurella multocida subsp. multocida infection in a closed colony of rabbits: characteristics of isolates. Lab Anim 25:236-41
DiGiacomo, R F; Taylor, F G; Allen, V et al. (1990) Naturally acquired Pasteurella multocida infection in rabbits: immunological aspects. Lab Anim Sci 40:289-92
Deeb, B J; DiGiacomo, R F; Bernard, B L et al. (1990) Pasteurella multocida and Bordetella bronchiseptica infections in rabbits. J Clin Microbiol 28:70-5
Deeb, B J; DiGiacomo, R F; Bernard, B L et al. (1989) Field trial of a live streptomycin dependent Pasteurella multocida serotype A:12 vaccine in rabbits. Lab Anim Sci 39:229-33
DiGiacomo, R F; Deeb, B J; Giddens Jr, W E et al. (1989) Atrophic rhinitis in New Zealand white rabbits infected with Pasteurella multocida. Am J Vet Res 50:1460-5
DiGiacomo, R F; Deeb, B J; Bernard, B L et al. (1987) Safety and efficacy of a streptomycin dependent live Pasteurella multocida vaccine in rabbits. Lab Anim Sci 37:187-90
DiGiacomo, R F; Jones, C D; Wathes, C M (1987) Transmission of Pasteurella multocida in rabbits. Lab Anim Sci 37:621-3
Klaassen, J M; Bernard, B L; DiGiacomo, R F (1985) Enzyme-linked immunosorbent assay for immunoglobulin G antibody to Pasteurella multocida in rabbits. J Clin Microbiol 21:617-21