The development of Retrovirus and Herpes B virus Specific Pathogen Free (SPF), rhesus macaques has become a primary goal in captive breeding programs. These SPF colonies are important for the conduct of AIDS research and in protecting the health of personnel who care for animals or handle animal tissue. The goal of this project is to develop technology that will permit the monitoring of genetic diversity in the SPF rhesus macaque breeding colonies. Rhesus macaque nuclear DNA will be examined with restriction enzymes and clones to identify hypervariable restriction fragment length polymorphisms, (RFLP). Three large group housed populations of rhesus macaques with known paternity data will then be examined with RFLP analysis to determine the efficiency of hypervariable regions in defining pedigrees. At the same time protocols for use of different kinds of tissue samples such as unrefrigerated whole blood, saliva, hair, and skin will also be developed to extend the research applications of this technology. Once the technique is refined pedigree determination by RFLP analysis will be applied to SPF colony founder stock at the California Primate Research Center and offered as a service to other institutions to extend beyond the duration of this grant. A long term research goal of this project is to compare RFLP regions with medical, reproductive, mortality, and morbidity data on individuals to hopefully identify genetic markers for disease predisposition and/or reproductive performance.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
5R24RR005090-02
Application #
3451155
Study Section
Animal Resources Advisory Committee (AR)
Project Start
1988-09-30
Project End
1993-09-29
Budget Start
1989-09-30
Budget End
1990-09-29
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
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