This is a joint Harvard-Brandeis program project, with goals that include both methodological developments and cell-biological applications. The four major goals are: (1) advance methods for obtaining atomic models of large structure by docking and refining atomic models of the component parts in molecular maps from electron cryomicroscopy (cryoEM); (2) improve the accuracy and resolution of the molecular maps we can obtain from cryoEM of single particles, accelerate 2D crystal structure determination, through development of molecular replacement and phase extension methods; (4) improve use of molecular maps from cryoEM to initiate phase determination in studies of large assemblies. By x-ray crystallography. There are four major projects, three cores, and a set of associated projects. The major and associated projects focus on the following biological systems: (1) the actin cytoskeleton; (2) viruses and viral entry into cells; (3) membrane proteins, especially members of the MIP and MAPEG families; (4) antibiotic peptide synthetases; (5) spliceosome assemblies; (6) the phase T7 replication complex, (""""""""replisome""""""""). The cores, which are a central component of the collaborative effort, support shared state-of-the-art electron microscope facilities, software development, and administration of the consortium.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Education Projects (R25)
Project #
1R25GM062584-01
Application #
6259469
Study Section
Special Emphasis Panel (ZGM1-MBRS-9 (SD))
Program Officer
Toliver, Adolphus
Project Start
2001-03-01
Project End
2005-02-28
Budget Start
2001-03-01
Budget End
2002-02-28
Support Year
1
Fiscal Year
2001
Total Cost
$308,406
Indirect Cost
Name
University of Arizona
Department
Type
Other Domestic Higher Education
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Guzman, Lindsay E; Kimani, Flora W; Jewett, John C (2016) Protecting Triazabutadienes To Afford Acid Resistance. Chembiochem 17:2220-2222
Boitano, Scott; Hoffman, Justin; Flynn, Andrea N et al. (2015) The novel PAR2 ligand C391 blocks multiple PAR2 signalling pathways in vitro and in vivo. Br J Pharmacol 172:4535-4545
Alt, Mary; Meyers, Christina; Oglivie, Trianna et al. (2014) Cross-situational statistically based word learning intervention for late-talking toddlers. J Commun Disord 52:207-20
Melemedjian, Ohannes K; Tillu, Dipti V; Asiedu, Marina N et al. (2013) BDNF regulates atypical PKC at spinal synapses to initiate and maintain a centralized chronic pain state. Mol Pain 9:12
Parker, Sara S; Mandell, Edward K; Hapak, Sophie M et al. (2013) Competing molecular interactions of aPKC isoforms regulate neuronal polarity. Proc Natl Acad Sci U S A 110:14450-5
Tsukada, Takehiro; Kotlyanskaya, Lucy; Huynh, Robert et al. (2011) Identification of residues within tropomodulin-1 responsible for its localization at the pointed ends of the actin filaments in cardiac myocytes. J Biol Chem 286:2194-204