Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Unknown (R35)
Project #
5R35CA044355-10
Application #
2091445
Study Section
Special Emphasis Panel (SRC (88))
Project Start
1987-07-01
Project End
2001-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204
Arvizu-Flores, Aldo A; Sugich-Miranda, Rocio; Arreola, Rodrigo et al. (2008) Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: calorimetric and crystallographic analysis of the K48Q mutant. Int J Biochem Cell Biol 40:2206-17
Saxl, Ruth L; Reston, James; Nie, Zhe et al. (2003) Modification of Escherichia coli thymidylate synthase at tyrosine-94 by 5-imidazolylpropynyl-2'-deoxyuridine 5'-monophosphate. Biochemistry 42:4544-51
Gribaudo, Giorgio; Riera, Ludovica; Caposio, Patrizia et al. (2003) Human cytomegalovirus requires cellular deoxycytidylate deaminase for replication in quiescent cells. J Gen Virol 84:1437-41
Johnson, Eric F; Hinz, Wolfgang; Atreya, Chloe E et al. (2002) Mechanistic characterization of Toxoplasma gondii thymidylate synthase (TS-DHFR)-dihydrofolate reductase. Evidence for a TS intermediate and TS half-sites reactivity. J Biol Chem 277:43126-36
Aschele, C; Debernardis, D; Bandelloni, R et al. (2002) Thymidylate synthase protein expression in colorectal cancer metastases predicts for clinical outcome to leucovorin-modulated bolus or infusional 5-fluorouracil but not methotrexate-modulated bolus 5-fluorouracil. Ann Oncol 13:1882-92
Saxl, R L; Changchien, L M; Hardy, L W et al. (2001) Parameters affecting the restoration of activity to inactive mutants of thymidylate synthase via subunit exchange: further evidence that thymidylate synthase is a half-of-the-sites activity enzyme. Biochemistry 40:5275-82
Almog, R; Waddling, C A; Maley, F et al. (2001) Crystal structure of a deletion mutant of human thymidylate synthase Delta (7-29) and its ternary complex with Tomudex and dUMP. Protein Sci 10:988-96
Changchien, L M; Garibian, A; Frasca, V et al. (2000) High-level expression of Escherichia coli and Bacillus subtilis thymidylate synthases. Protein Expr Purif 19:265-70
Keefe, R G; Maley, G F; Saxl, R L et al. (2000) A T4-phage deoxycytidylate deaminase mutant that no longer requires deoxycytidine 5'-triphosphate for activation. J Biol Chem 275:12598-602
Aschele, C; Debernardis, D; Tunesi, G et al. (2000) Thymidylate synthase protein expression in primary colorectal cancer compared with the corresponding distant metastases and relationship with the clinical response to 5-fluorouracil. Clin Cancer Res 6:4797-802

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