Abstract

We propose to continue and extend our studies on molecular events leading to poliovirus replication and our analysis of neutralization antigenic sites of the virus particle. Specifically, we propose to analyze the structure and function(s) of the 5' non- coding region of poliovirus RNA, to determine processes of protein modification and of proteolytic cleavage of the poliovirus polyprotein, to investigate the structure and substrate recognition of the two virus-encoded proteinases, and to analyze components of viral RNA replication and the mechanism of initiation of viral RNA synthesis. Moreover, we will alter the surface structure of the poliovirion to achieve hybrid virus expressing foreign antigenic determinants in addition to those of poliovirus Type 1 (Mahoney). Our studies may lead to the development of new vaccines and of novel drugs directed against picornavirus infection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI015122-18
Application #
3480822
Study Section
Experimental Virology Study Section (EVR)
Project Start
1978-04-01
Project End
1993-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
18
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Song, Yutong; Gorbatsevych, Oleksandr; Liu, Ying et al. (2017) Limits of variation, specific infectivity, and genome packaging of massively recoded poliovirus genomes. Proc Natl Acad Sci U S A 114:E8731-E8740
Asare, Emmanuel; Mugavero, JoAnn; Jiang, Ping et al. (2016) A Single Amino Acid Substitution in Poliovirus Nonstructural Protein 2CATPase Causes Conditional Defects in Encapsidation and Uncoating. J Virol 90:6174-6186
Chen, Ying-Han; Du, WenLi; Hagemeijer, Marne C et al. (2015) Phosphatidylserine vesicles enable efficient en bloc transmission of enteroviruses. Cell 160:619-630
Jiang, Ping; Liu, Ying; Ma, Hsin-Chieh et al. (2014) Picornavirus morphogenesis. Microbiol Mol Biol Rev 78:418-37
Wang, Chunling; Ma, Hsin-Chieh; Wimmer, Eckard et al. (2014) A C-terminal, cysteine-rich site in poliovirus 2C(ATPase) is required for morphogenesis. J Gen Virol 95:1255-65
Ma, Hsin-Chieh; Liu, Ying; Wang, Chunling et al. (2014) An interaction between glutathione and the capsid is required for the morphogenesis of C-cluster enteroviruses. PLoS Pathog 10:e1004052
Schein, Catherine H; Bowen, David M; Lewis, Jessica A et al. (2012) Physicochemical property consensus sequences for functional analysis, design of multivalent antigens and targeted antivirals. BMC Bioinformatics 13 Suppl 13:S9
Wang, Chunling; Jiang, Ping; Sand, Claire et al. (2012) Alanine scanning of poliovirus 2CATPase reveals new genetic evidence that capsid protein/2CATPase interactions are essential for morphogenesis. J Virol 86:9964-75
Song, Yutong; Paul, Aniko V; Wimmer, Eckard (2012) Evolution of poliovirus defective interfering particles expressing Gaussia luciferase. J Virol 86:1999-2010
Toyoda, Hidemi; Wimmer, Eckard; Cello, Jeronimo (2011) Oncolytic poliovirus therapy and immunization with poliovirus-infected cell lysate induces potent antitumor immunity against neuroblastoma in vivo. Int J Oncol 38:81-7

Showing the most recent 10 out of 147 publications