Abstract

Program Director/Principal Investigator (Last, First, Middle): M c M a h o n , A n d r e w PROJECT SUMMA,RY (See instotctions): The mammalian kidney has a rich history of embryological study, its physiological actions are well understood and kidney disease is one of the leading health care issues. For these and other reasons (for example the (dynamic cellular events intrinsic to the kidney program), we have chosen to focus on this system. As we wish to relate our findings as directly as possible to the clinic, the mouse is our rnodel. Combining genetic, genomic and biochemical strategies, the proposed research will define and mechanistically dissect key regulatory processes in the developing mammalian kidney.

Public Health Relevance

Our goal is do determine how nephron generating stem cells are regulated to enable new approaches to treat kidney disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37DK054364-18
Application #
8919874
Study Section
Special Emphasis Panel (NSS)
Program Officer
Hoshizaki, Deborah K
Project Start
1998-09-01
Project End
2018-08-31
Budget Start
2015-09-01
Budget End
2016-08-31
Support Year
18
Fiscal Year
2015
Total Cost
$744,632
Indirect Cost
$293,340

  Institution

Name
University of Southern California
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089

  Publications

Przepiorski, Aneta; Sander, Veronika; Tran, Tracy et al. (2018) A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells. Stem Cell Reports 11:470-484
O'Brien, Lori L; Combes, Alexander N; Short, Kieran M et al. (2018) Wnt11 directs nephron progenitor polarity and motile behavior ultimately determining nephron endowment. Elife 7:
Ramalingam, Harini; Fessler, Alicia R; Das, Amrita et al. (2018) Disparate levels of beta-catenin activity determine nephron progenitor cell fate. Dev Biol 440:13-21
Rutledge, Elisabeth A; Benazet, Jean-Denis; McMahon, Andrew P (2017) Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development. Development 144:3177-3188
Oxburgh, Leif; Carroll, Thomas J; Cleaver, Ondine et al. (2017) (Re)Building a Kidney. J Am Soc Nephrol 28:1370-1378
Naiman, Natalie; Fujioka, Kaoru; Fujino, Mari et al. (2017) Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development. Dev Cell 41:349-365.e3
O'Brien, Lori L; Guo, Qiuyu; Lee, YoungJin et al. (2016) Differential regulation of mouse and human nephron progenitors by the Six family of transcriptional regulators. Development 143:595-608
Li, Joan; Ariunbold, Usukhbayar; Suhaimi, Norseha et al. (2015) Collecting duct-derived cells display mesenchymal stem cell properties and retain selective in vitro and in vivo epithelial capacity. J Am Soc Nephrol 26:81-94
Kobayashi, Akio; Mugford, Joshua W; Krautzberger, A Michaela et al. (2014) Identification of a multipotent self-renewing stromal progenitor population during mammalian kidney organogenesis. Stem Cell Reports 3:650-62
Short, Kieran M; Combes, Alexander N; Lefevre, James et al. (2014) Global quantification of tissue dynamics in the developing mouse kidney. Dev Cell 29:188-202

Showing the most recent 10 out of 33 publications