Abstract

In order to facilitate the ultimate production of a diagnostic and vaccine for human viral NANB hepatitis using recombinant DNA technology, strategies designed to isolate viral nucleic acid will be initiated through the production of enriched recombinant cDNA libraries and by direct radio-labeling studies. In addition, monoclonal antibodies will be made against virus preparations and an in vitro system for propagating virus investigated. An association of reverse transcriptase activity with virus will also be investigated. Progress in these areas should lead to the molecular cloning, characterization and recombinant expression of the virus genome which in turn will allow diagnostic development and subsequent vaccine approaches.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43AI022774-01
Application #
3488382
Study Section
(SSS)
Project Start
1985-09-01
Project End
1986-02-28
Budget Start
1985-09-01
Budget End
1986-02-28
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Protos Corporation
Department
Type
DUNS #
City
Emeryville
State
CA
Country
United States
Zip Code
94608

  Publications

Cox, Gary M; Harrison, Thomas S; McDade, Henry C et al. (2003) Superoxide dismutase influences the virulence of Cryptococcus neoformans by affecting growth within macrophages. Infect Immun 71:173-80
Feldmesser, Marta; Mednick, Aron; Casadevall, Arturo (2002) Antibody-mediated protection in murine Cryptococcus neoformans infection is associated with pleotrophic effects on cytokine and leukocyte responses. Infect Immun 70:1571-80
Nosanchuk, J D; Ovalle, R; Casadevall, A (2001) Glyphosate inhibits melanization of Cryptococcus neoformans and prolongs survival of mice after systemic infection. J Infect Dis 183:1093-9
Feldmesser, M; Kress, Y; Casadevall, A (2001) Dynamic changes in the morphology of Cryptococcus neoformans during murine pulmonary infection. Microbiology 147:2355-65
Cox, G M; McDade, H C; Chen, S C et al. (2001) Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans. Mol Microbiol 39:166-75
Feldmesser, M; Kress, Y; Mednick, A et al. (2000) The effect of the echinocandin analogue caspofungin on cell wall glucan synthesis by Cryptococcus neoformans. J Infect Dis 182:1791-5
Cox, G M; Mukherjee, J; Cole, G T et al. (2000) Urease as a virulence factor in experimental cryptococcosis. Infect Immun 68:443-8
Feldmesser, M; Kress, Y; Novikoff, P et al. (2000) Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect Immun 68:4225-37
MacGill, T C; MacGill, R S; Casadevall, A et al. (2000) Biological correlates of capsular (quellung) reactions of Cryptococcus neoformans. J Immunol 164:4835-42
Franzot, S P; Salkin, I F; Casadevall, A (1999) Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J Clin Microbiol 37:838-40

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