The goal of this proposal is to develop a system by which cloned gene products can be efficiently secreted from the E. coli host bacterium into the media. This system would offer significant advantages to conventional E. coli expression systems since it would permit the isolation of products previously observed to be either lethal to the host or subject to rapid proteolytic degradation. Furthermore, by remaining within E. coli, the savings in time, effort, and cost of cloning a gene and expressing its product would be considerable.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43AI035523-01
Application #
2071248
Study Section
Special Emphasis Panel (ZRG7-SSS-2 (08))
Project Start
1994-09-30
Project End
1995-03-31
Budget Start
1994-09-30
Budget End
1995-03-31
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Stratagene
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92037