Monolayer, non-differentiated tracheo-bronchial epithelial cell cultures have been helpful in furthering the understanding of diseases such as cystic fibrosis and asthma, characterizing viral infection in the airway epithelia, and advancing our knowledge of tracheal and bronchial inflammation. In addition, such cultures have enabled and/or facilitated inhalation toxicology studies and allowed insight into neoplastic progression in the airways. Differentiated, stratified cultures which adopt an in vivo-like phenotype are anticipated to add further to these advances. Nonetheless, a differentiated, commercially available model of the airway epithelia does not exist. Phase I research will investigate the feasibility of producing a tissue culture model of the airway epithelia which mimics its in vivo progenitors in structure and function. Normal, human derived, tracheobronchial epithelial cells will be expanded in monolayer culture, cryopreserved, and then used to produce stratified, differentiated tissue cultures. Experiments will investigate a variety of culture parameters and cultures will be characterized using microscopic and immunospecific techniques. Finally, the technical and commercial viability of the model will be assessed.

Proposed Commercial Applications

The differentiated, tissue culture model here proposed would facilitate the screening and testing of new pharmacologic agents and therapies to combat the various diseases and disorders affecting the airway epithelia. In addition, an organotypic tissue model would be useful for toxicology, cancer, and drug deliver studies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43DK052245-01
Application #
2017769
Study Section
Special Emphasis Panel (ZRG2-SSS-3 (37))
Program Officer
Mckeon, Catherine T
Project Start
1997-01-01
Project End
1997-06-30
Budget Start
1997-01-01
Budget End
1997-06-30
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Mattek Corporation
Department
Type
DUNS #
City
Ashland
State
MA
Country
United States
Zip Code
01721