Confocal microscopy is one of the most significant advances in optical microscopy. Its ability to virtually eliminate out-of-focus blurring permits optical sectioning of cells and thick transparent specimens. This technique is rapidly being adopted for the three-dimensional visualization of cellular and tissue organization.
The specific aims of this proposal are to determine the feasibility of using a slit scanning method and a CCD camera to produce optical sections of fluorescently stained biological specimens. The long term objective is to provide a rapid, low-cost confocal imaging instrument that can be attached to most conventional epi-illumination microscopes. To this end, an optical breadboard will be developed that incorporates a unique scanning device that simultaneously scans a specimen and a CCD camera. The simplicity of this design is expected to reduce the cost of the system, and the use of a CCD camera will provide the flexibility for confocal and conventional video imaging. The combination of these innovations will result in an affordable confocal microscope that can be used for research in cell biology, neurobiology, cancer, histology, pathology and molecular genetics. In addition, the cost of this instrument should make this technology more available for clinical and instructional applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43GM045640-01
Application #
3498592
Study Section
Special Emphasis Panel (SSS (B3))
Project Start
1991-02-01
Project End
1991-05-31
Budget Start
1991-02-01
Budget End
1991-05-31
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Meridian Instruments, Inc.
Department
Type
DUNS #
City
Okemos
State
MI
Country
United States
Zip Code
48864