The main objective of this project is to develop a platform technology, Nanopore Enhanced Enzyme Device (NEED), for the production of enantiomerically pure (i.e. chiral specific) drug intermediates. The enzyme is to be immobilized on a nanopore support whose pore morphology and surface modifications will be specifically designed for further enhancing the enzyme's activity and selectivity.
The specific aims of this project are: A.1 Lipase Immobilization with nanopore silica-chitosan composite as a bioreactor A.2 Enhancing Enzyme Performances by modifying nanopore support and controlling water activity A.3 Achieving Reaction and Separation Synergistically in NEED In accordance with the three specific aims set above, we propose to accomplish the following technical milestones during the course of this project: (1) Immobilizing a lipase onto silica-chitosan nanopore composite while maintaining its activity and selectivity; (2) Creating open channel structure and high surface area in the nanocomposite through engineered pore structures to significantly enhance the reaction rate of the attached lipase; (3) Increasing the hydrophobic-hydrophilic interfacial area, by incorporating surface modification ligands, within the composite to further enhance lipase's activity; (4) Using the swelling ratio of the chitosan phase to control and fine tune water activity surrounding the lipase; (5) Enhancing the efficiencies of reaction as well as separation by selectively removing a product in a continuous flow reactor; (6) Accomplishing simultaneous chiral production and separation in a bioreactor composed of lipase beads modified with chiral specific ligand(s). Candida antarctica lipase B (CALB) will be immobilized onto nanopore silica-chitosan composite for possible productions, in replacements of traditional productions using sophisticated synthesis and separation schemes, of fine chemicals to be used by pharmaceutical, food/nutrition, LCD industries. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43GM077711-01
Application #
7108783
Study Section
Special Emphasis Panel (ZRG1-CB-A (10))
Program Officer
Jones, Warren
Project Start
2006-04-01
Project End
2006-12-31
Budget Start
2006-04-01
Budget End
2006-12-31
Support Year
1
Fiscal Year
2006
Total Cost
$173,344
Indirect Cost
Name
Industrial Science & Technology Network
Department
Type
DUNS #
159817535
City
York
State
PA
Country
United States
Zip Code
17404