In this proposal, Vuronyx Technologies in collaboration with Prof. Vince Rotello at UMass Amherst aims to develop a prototype kit for validation of cell lines. Cell lines have a wide range of applications in both academic and life sciences industries, including genomics, drug discovery, tissue engineering, regenerative medicine, and toxicity testing. However, cell cultures are prone to contamination by foreign cells, which may rapidly displace the original cells. The identity of cultured cells should be routinely verified, but a majority of laboratories do not monitor the identity of their cell lines, and many cell lines are misidentified. The costs, effort and time required to confirm the identity of cell lines has been a barrier to adoption of cell line identification as a routine quality control measure Our preliminary results show that our pattern-based approach is capable of distinguishing both cell genotype and phenotype. Our research will focus on developing a ?minimalist? polymer- only sensor array that can rapidly discriminate between cell types and geno/phenotypes. The polymer platform presents a scalable and reproducible platform, an advantage that will be furthered through close collaboration between Rotello and Vuronyx. The objective in Phase 1 is to develop the polymer nanoparticle based cell sensing strategy and test it against a range of cell types. This proposal is directly relevant to NIH?s mission of fostering creative discoveries for human well-being, as well as NIGMS?s mission of understanding of biological processes and advancing disease diagnosis, treatment and prevention.

Public Health Relevance

In this proposal, Vuronyx Technologies in collaboration with Prof. Vince Rotello at UMass Amherst aims to develop a prototype kit for validation of cell lines. Our preliminary results show that our pattern-based approach is capable of distinguishing both cell genotype and phenotype. The key challenge with these systems is scalability: the cost of producing nanoparticles and proteins would make the above systems uncompetitive on the market. Our research will focus on developing a ?minimalist? polymer-only sensor array that can rapidly discriminate between cell types and geno/phenotypes. The polymer platform presents a scalable and reproducible platform, an advantage that will be furthered through close collaboration between Rotello and Vuronyx.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43GM123868-01A1
Application #
9406682
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Marino, Pamela
Project Start
2017-09-01
Project End
2018-08-31
Budget Start
2017-09-01
Budget End
2018-08-31
Support Year
1
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Vuronyx Technologies, LLC
Department
Type
DUNS #
967586947
City
Medford
State
MA
Country
United States
Zip Code
02155