The primary objective of this proposal is to develop a novel ion source for the efficient on-line coupling of a wide array of chromatographic techniques with mass spectrometry. The use of liquid chromatography separation is a prerequisite for high-throughput mass spectrometry analysis of complex proteomics samples. Commonly used electrospray ionization (ESI), although allowing a direct coupling, imposes significant limitations on the mobile phases and additives used in LC separations. The most widely used separation technique, reversed-phase liquid chromatography, is performed in non-optimal conditions to satisfy operational requirements of the electrospray ion source. The proposed ion source will overcome these limitations and provide efficient analyte ionization from a wide range of liquid sample compositions. Thus, the ion source will allow RP-LC to perform in optimal conditions and also the use of chromatographic techniques not previously amenable by direct ESI-MS analysis.

Public Health Relevance

Proteomics studies biological processes by the systematic analysis of proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high-throughput technology for the efficient on- line coupling of a wide array of chromatographic separation techniques with mass spectrometry.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43RR031942-01
Application #
8057752
Study Section
Special Emphasis Panel (ZRG1-IMST-G (10))
Program Officer
Sheeley, Douglas
Project Start
2011-03-01
Project End
2013-02-28
Budget Start
2011-03-01
Budget End
2013-02-28
Support Year
1
Fiscal Year
2011
Total Cost
$200,000
Indirect Cost
Name
Masstech, Inc.
Department
Type
DUNS #
123310083
City
Columbia
State
MD
Country
United States
Zip Code
21046