The long-term objective of these studies is to define in sensory cortex, the differential physiological roles of subcortical neurotransmitter/neuromodulator (neurotransmitter) systems which project in a diffuse manner to neocortex (i.e. acetylcholine (ACh), norepinephrine (NE), serotonin and dopamine); the proposed studies will focus on the pattern of conditioning-related ACh and NE release in auditory cortex. These studies should be viewed as preliminary; however, data from the proposed experiments should aid in providing a basis for understanding the cognitive and memory alterations associated with age- and/or disease-related alterations of neurotransmitter function in cortex. Such information is prerequisite to the rational design of therapeutic agents for use in Alzheimer's Disease and related disorders where cortical neurotransmitter function is compromised.
The specific aims of the proposed studies are to: 1. Characterize the effect of sensitization training, associative conditioning, extinction and re-development on ACh release in auditory cortex. 2. Initiate studies to determine the pattern(s) of conditioning-related release of biogenic amines in rat auditory cortex. 3. Provide a laboratory environment to be used in the training of potential neuroscientists, with an emphasis on under-represented minorities. All experiments will be conducted in the urethane anesthetized rat. Pure tones will be paired with rewarding medial forebrain bundle (MFB) stimulation for conditioning. ACh release will be sampled in auditory cortex ipsilateral to MFB stimulation with microdialysis and analyzed with HPLC and electrochemical detection. Multi-unit neuronal activity will be recorded with low resistance glass microelectrodes. NE will be measured using in-situ electrochemistry.

Project Start
1997-08-01
Project End
1998-07-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
26
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Meharry Medical College
Department
Type
DUNS #
City
Nashville
State
TN
Country
United States
Zip Code
37208
Pulliam, Stephanie R; Pellom Jr, Samuel T; Shanker, Anil et al. (2016) Butyrate regulates the expression of inflammatory and chemotactic cytokines in human acute leukemic cells during apoptosis. Cytokine 84:74-87
Chen, Chau-Kuang; Bruce, Michelle; Tyler, Lauren et al. (2013) Analysis of an environmental exposure health questionnaire in a metropolitan minority population utilizing logistic regression and Support Vector Machines. J Health Care Poor Underserved 24:153-71
Boadi, William Y; Harris, Shalandus; Anderson, Justin B et al. (2013) Lipid peroxides and glutathione status in human progenitor mononuclear (U937) cells following exposure to low doses of nickel and copper. Drug Chem Toxicol 36:155-62
Choudhury, Shahana A; Ladson, Gwinnett; Kabir, Madina S (2012) Evaluation of serotype-specific immunity to Streptococcus pneumoniae in pregnant women and cord blood of infants: impact of race and ethnicity. J Natl Med Assoc 104:251-7
Hall 3rd, Mack; Misra, Smita; Chaudhuri, Minu et al. (2011) Peptide aptamer mimicking RAD51-binding domain of BRCA2 inhibits DNA damage repair and survival in Trypanosoma brucei. Microb Pathog 50:252-62
Kawai, Yumiko; Garduño, Lakisha; Theodore, Melanie et al. (2011) Acetylation-deacetylation of the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) regulates its transcriptional activity and nucleocytoplasmic localization. J Biol Chem 286:7629-40
Rana, Tanu; Misra, Smita; Mittal, Mukul K et al. (2011) Mechanism of down-regulation of RNA polymerase III-transcribed non-coding RNA genes in macrophages by Leishmania. J Biol Chem 286:6614-26
Farrow, Anitra L; Rana, Tanu; Mittal, Mukul K et al. (2011) Leishmania-induced repression of selected non-coding RNA genes containing B-box element at their promoters in alternatively polarized M2 macrophages. Mol Cell Biochem 350:47-57
Sharma, Shvetank; Singha, Ujjal K; Chaudhuri, Minu (2010) Role of Tob55 on mitochondrial protein biogenesis in Trypanosoma brucei. Mol Biochem Parasitol 174:89-100
Sheng, Liu; Ding, Xinxin; Ferguson, Marcus et al. (2010) Prenatal polycyclic aromatic hydrocarbon exposure leads to behavioral deficits and downregulation of receptor tyrosine kinase, MET. Toxicol Sci 118:625-34

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