Abstract

Excessive extracellular matrix metalloproteinases (MMPs) activities in the cornea can lead to cornea ulceration. Cicatrization due to cornea ulceration is a major cause of blindness and impaired vision. We are concerned about the effect of commonly used eye medications and UV radiation on corneal MMPs. We propose to study the four major corneal MMPs: gelatinase A, gelatinase B, collagenase and stromelysin under normal, injured and diseased conditions in organ culture. Pig corneas will be used. The effect of eye medications and UV radiation on the net synthesis of each of these MMPs will be evaluated. The medications to be tested include agents for antibacterial, antifungus, antihistamine, pupil dilator, antiglucoma, anticholinergic and anti-inflammatory. The effect of these medications of the activity of each purified MMPs will also be studied. The results of our work may lead to a better understanding of these medications and UV radiation on the structural integrity of the cornea. Our long term goal is to determine the effect of environmental agents on the structural integrity of the cornea.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
3S06GM008047-25S1
Application #
6296619
Study Section
Project Start
1998-07-01
Project End
1999-06-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
25
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Jackson State University
Department
Type
DUNS #
044507085
City
Jackson
State
MS
Country
United States
Zip Code
39217

  Publications

Beqa, Lule; Fan, Zhen; Singh, Anant Kumar et al. (2011) Gold nano-popcorn attached SWCNT hybrid nanomaterial for targeted diagnosis and photothermal therapy of human breast cancer cells. ACS Appl Mater Interfaces 3:3316-24
Khan, Sadia Afrin; Singh, Anant K; Senapati, Dulal et al. (2011) Targeted highly sensitive detection of multi-drug resistant Salmonella DT104 using gold nanoparticles. Chem Commun (Camb) 47:9444-6
Singh, Anant K; Lu, Wentong; Senapati, Dulal et al. (2011) Long-range nanoparticle surface-energy-transfer ruler for monitoring photothermal therapy response. Small 7:2517-25
Huang, Yong; Zhao, Shulin; Shi, Ming et al. (2010) Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis. Anal Biochem 399:72-7
Wang, Shuguang; Singh, Anant K; Senapati, Dulal et al. (2010) Rapid colorimetric identification and targeted photothermal lysis of Salmonella bacteria by using bioconjugated oval-shaped gold nanoparticles. Chemistry 16:5600-6
Lu, Wentong; Singh, Anant Kumar; Khan, Sadia Afrin et al. (2010) Gold nano-popcorn-based targeted diagnosis, nanotherapy treatment, and in situ monitoring of photothermal therapy response of prostate cancer cells using surface-enhanced Raman spectroscopy. J Am Chem Soc 132:18103-14
Lu, Wentong; Arumugam, Sri Ranjini; Senapati, Dulal et al. (2010) Multifunctional oval-shaped gold-nanoparticle-based selective detection of breast cancer cells using simple colorimetric and highly sensitive two-photon scattering assay. ACS Nano 4:1739-49
Dadiboyena, Sureshbabu; Valente, Edward J; Hamme 2nd, Ashton T (2010) Synthesis of Novel Pyrazoles via [2+3]-Dipolar Cycloaddition Using Alkyne Surrogates. Tetrahedron Lett 51:1341
Stewart, Gernerique; Smith, Keonia; Chornes, Ashley et al. (2010) PHOTOCHEMICAL REACTION OF NITRO-POLYCYCLIC AROMATIC HYDROCARBONS: EFFECT BY SOLVENT AND STRUCTURE. Environ Chem Lett 8:301-306
Zhao, Shulin; Huang, Yong; Shi, Ming et al. (2010) Chemiluminescence resonance energy transfer-based detection for microchip electrophoresis. Anal Chem 82:2036-41

Showing the most recent 10 out of 119 publications