This research will focus on molecular labeling of secretory vesicles and cytoskeletal elements involved in the secretion of adhesive material and wall components to the surface of fungal cysts and hyphal tips. The study should provide-a clearer understanding of the role of an assortment of vacuoles, vesicles and cisternae which develop at specific times in the life cycle of these fungi and a clearer picture of the role which microfilaments and microtubules play in guiding these vesicles to sites of secretion. Specifically, the developmental pathway of a prominent secretory vesicle, the """"""""peripheral vesicle"""""""", will be followed from synthesis to secretion. Gold-labeled lectins will be used to label thin sections prepared for transmission electron microscopy to trace the development of the vesicles in germinating cysts and oospores. Neither of these synthesized upon germination. Samples of germinating spores will be fixed at selected intervals for transmission electron microscopy, stained with gold labeled lectins, and examined for the involvement of the Golgi apparatus, endoplasmic reticulum, or other organelles in the synthesis of these vesicles. As the peripheral vesicles migrate to the periphery of zoospores of these fungi, the exocytosis of adhesive material to the cell surface or the internalization of these vesicles will again be monitored with gold labeled lectins and transmission electron microscopy. Finally, rhodamine conjugated phalloidin and fluorescein-labeled antibodies will be used to visualize microfilaments and microtubules of the cytoskeleton, respectively. Fluorescence microscopy will show the involvement of the cytoskeleton in directed cellular secretion and may tie in with the role of calcium in stimulating encystment and hyphal tip growth in these fungi. This project will provide participating students with hands-on experience with modern molecular techniques for localizing cellular components and cytoskeletal elements.

Project Start
Project End
Budget Start
Budget End
Support Year
24
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Hawaii at Hilo
Department
Type
DUNS #
965088057
City
Hilo
State
HI
Country
United States
Zip Code
96720
Mishra, Gita D; Chung, Hsin-Fang; Pandeya, Nirmala et al. (2016) The InterLACE study: Design, data harmonization and characteristics across 20 studies on women's health. Maturitas 92:176-185
Morrison, Lynn A; Brown, Daniel E; Sievert, Lynnette L et al. (2014) Voices from the Hilo Women's Health Study: talking story about menopause. Health Care Women Int 35:529-48
Sievert, Lynnette Leidy; Murphy, Lorna; Morrison, Lynn A et al. (2013) Age at menopause and determinants of hysterectomy and menopause in a multi-ethnic community: the Hilo Women's Health Study. Maturitas 76:334-41
Van Berge-Landry, Helene; James, Gary D; Brown, Daniel E (2013) Relationships between diurnal changes in blood pressure and catecholamines among Filipino-American and European-American women. Am J Hum Biol 25:431-3
Reza, Angela; Sievert, Lynnette Leidy; Rahberg, Nichole et al. (2012) Prevalence and determinants of headaches in Hawaii: the Hilo Women's Health Study. Ann Hum Biol 39:305-14
Stringer, Kate; Kerpelman, Jennifer; Skorikov, Vladimir (2012) A longitudinal examination of career preparation and adjustment during the transition from high school. Dev Psychol 48:1343-54
Brown, Daniel E; Sievert, Lynnette L; Morrison, Lynn A et al. (2011) Relationship between hot flashes and ambulatory blood pressure: the Hilo women's health study. Psychosom Med 73:166-72
Morrison, Lynn A; Larkspur, Louona; Calibuso, Marites J et al. (2010) Women's attitudes about menstruation and associated health and behavioral characteristics. Am J Health Behav 34:90-100
Morrison, Lynn A; Sievert, Lynnette L; Brown, Daniel E et al. (2010) Relationships between menstrual and menopausal attitudes and associated demographic and health characteristics: the Hilo Women's Health Study. Women Health 50:397-413
Sievert, Lynnette L; Reza, Angela; Mills, Phoebe et al. (2010) Diurnal rhythm and concordance between objective and subjective hot flashes: the Hilo Women's Health Study. Menopause 17:471-9

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