Abstract

The immediate goal of this research is to study the thermal unfolding of two biomedically important proteins: apolipoprotein (a) and porcine pancreatic lipase at various pH values, solvent conditions and protein concentrations. Apolipoprotein (a), a cholesterol-ester/LDL-like particle was selected for analysis because it has been found to be highly atherogenic (that is, associated with atherosclerotic cardiovascular disease) and has a fascinating structure composed of 17 almost identical domains. At the carboxy terminus, apolipoprotein (a) has single copies of sequences resembling the protease domain of plasminogen. Apolipoprotein (a) is not commercially available but milligram quantities of it are available to us from a confirmed source. Porcine pancreatic lipase has been selected for analysis because of its function; which is to catalyze the hydrolysis of ester bonds of nonpolar dietary long-chain triglycerides. Students will be trained in protein purification techniques and will (1) purify pancreatic lipase from the pancreas of freshly slaughtered hogs, (2) characterize pancreatic lipase using enzyme assays and biochemical methods and (3) study the nature of the proteins' thermal unfolding using differential scanning calorimetry.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008111-22
Application #
3755891
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
22
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Florida Agricultural and Mechanical University
Department
Type
DUNS #
City
Tallahassee
State
FL
Country
United States
Zip Code
32307

  Publications

Johnston, Jermaine G; Pollock, David M (2018) Circadian regulation of renal function. Free Radic Biol Med 119:93-107
Adams, Mark K; Lee, Seung-Ah; Belyaeva, Olga V et al. (2017) Characterization of human short chain dehydrogenase/reductase SDR16C family members related to retinol dehydrogenase 10. Chem Biol Interact 276:88-94
Mochona, Bereket; Jackson, Timothy; McCauley, DeCoria et al. (2016) Synthesis and Cytotoxic Evaluation of Pyrrole Hetarylazoles Containing Benzimidazole/Pyrazolone/1,3,4-Oxadiazole Motifs. J Heterocycl Chem 53:1871-1877
Engel, Krysta L; French, Sarah L; Viktorovskaya, Olga V et al. (2015) Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I. Mol Cell Biol 35:2321-31
Ayuk-Takem, Lambert; Amissah, Felix; Aguilar, Byron J et al. (2014) Inhibition of polyisoprenylated methylated protein methyl esterase by synthetic musks induces cell degeneration. Environ Toxicol 29:466-77
Chougule, Mahavir B; Patel, Apurva R; Patlolla, Ram et al. (2014) Epithelial transport of noscapine across cell monolayer and influence of absorption enhancers on in vitro permeation and bioavailability: implications for intestinal absorption. J Drug Target 22:498-508
Culpepper, Bonnie K; Webb, William M; Bonvallet, Paul P et al. (2014) Tunable delivery of bioactive peptides from hydroxyapatite biomaterials and allograft bone using variable-length polyglutamate domains. J Biomed Mater Res A 102:1008-16
Errahali, Younes J; Thomas, Leeshawn D; Keller 3rd, Thomas C S et al. (2013) Inhibition by new glucocorticoid antedrugs [16?, 17?-d] isoxazoline and [16?, 17?-d]-3'-hydroxy-iminoformyl isoxazoline derivatives of chemotaxis and CCL26, CCL11, IL-8, and RANTES secretion. J Interferon Cytokine Res 33:493-507
Stephenson, Adrienne P; Schneider, Jeffrey A; Nelson, Bryant C et al. (2013) Manganese-induced oxidative DNA damage in neuronal SH-SY5Y cells: attenuation of thymine base lesions by glutathione and N-acetylcysteine. Toxicol Lett 218:299-307
Godugu, Chandraiah; Patel, Apurva R; Doddapaneni, Ravi et al. (2013) Inhalation delivery of Telmisartan enhances intratumoral distribution of nanoparticles in lung cancer models. J Control Release 172:86-95

Showing the most recent 10 out of 40 publications