The active contractile components of skeletal muscle have and continue to be studied extensively. In contrast, passive connective tissue components have been neglected. This neglect is puzzling as disorders such as muscular dystrophy, myositis, ischemic muscular necrosis and others are associated with intramuscular connective tissue changes. Further, neural and vascular channels to the active components pass within the surrounding and very plastic connective tissue network.
the aims of this project are 1) to determine the changes in the quantity and composition of intramuscular connective tissue when muscles are stressed by over-use; 2) to correlate the changes in muscular connective tissue with twitch tension, tetanic tension and fatigability; 3) to determine if changes are age and load dependent; and 4) to establish a baseline for future studies involving pathological neuromuscular disorders. Juvenile (3-4 weeks) and adult (12-13 weeks) syrian hamsters will be subdivided randomly into a control group and two experimental groups. In experimental group I, muscle over-use will be accomplished by unilateral detachment of a synergist muscle in the leg. In experimental group II, muscle over-use in the leg muscles will be accomplished by applying subcutaneous loads in the lumbosacral region that are carried by the animals for 6 weeks. For all groups, (1) the percent composition of collagen, elastic and reticular fibers will be determined by serial histological procedures; (2) the quantity of connective tissue will be measured using computer digitizing techniques and (3) the physiological properties of the muscles determined and correlated with morphological changes. It is hypothesized that (1) tensile stresses in over-used muscles increase the quantity of intramuscular collagen, but not elastin or reticulin, increasing muscle stiffness but not maximum tetanic tension, (2) increased stiffness produces increased fatigability due to capillary compression, and (3) the magnitude of connective tissue changes are age dependent.
|Johnston, Jermaine G; Pollock, David M (2018) Circadian regulation of renal function. Free Radic Biol Med 119:93-107|
|Adams, Mark K; Lee, Seung-Ah; Belyaeva, Olga V et al. (2017) Characterization of human short chain dehydrogenase/reductase SDR16C family members related to retinol dehydrogenase 10. Chem Biol Interact 276:88-94|
|Mochona, Bereket; Jackson, Timothy; McCauley, DeCoria et al. (2016) Synthesis and Cytotoxic Evaluation of Pyrrole Hetarylazoles Containing Benzimidazole/Pyrazolone/1,3,4-Oxadiazole Motifs. J Heterocycl Chem 53:1871-1877|
|Engel, Krysta L; French, Sarah L; Viktorovskaya, Olga V et al. (2015) Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I. Mol Cell Biol 35:2321-31|
|Ayuk-Takem, Lambert; Amissah, Felix; Aguilar, Byron J et al. (2014) Inhibition of polyisoprenylated methylated protein methyl esterase by synthetic musks induces cell degeneration. Environ Toxicol 29:466-77|
|Chougule, Mahavir B; Patel, Apurva R; Patlolla, Ram et al. (2014) Epithelial transport of noscapine across cell monolayer and influence of absorption enhancers on in vitro permeation and bioavailability: implications for intestinal absorption. J Drug Target 22:498-508|
|Culpepper, Bonnie K; Webb, William M; Bonvallet, Paul P et al. (2014) Tunable delivery of bioactive peptides from hydroxyapatite biomaterials and allograft bone using variable-length polyglutamate domains. J Biomed Mater Res A 102:1008-16|
|Errahali, Younes J; Thomas, Leeshawn D; Keller 3rd, Thomas C S et al. (2013) Inhibition by new glucocorticoid antedrugs [16?, 17?-d] isoxazoline and [16?, 17?-d]-3'-hydroxy-iminoformyl isoxazoline derivatives of chemotaxis and CCL26, CCL11, IL-8, and RANTES secretion. J Interferon Cytokine Res 33:493-507|
|Stephenson, Adrienne P; Schneider, Jeffrey A; Nelson, Bryant C et al. (2013) Manganese-induced oxidative DNA damage in neuronal SH-SY5Y cells: attenuation of thymine base lesions by glutathione and N-acetylcysteine. Toxicol Lett 218:299-307|
|Godugu, Chandraiah; Patel, Apurva R; Doddapaneni, Ravi et al. (2013) Inhalation delivery of Telmisartan enhances intratumoral distribution of nanoparticles in lung cancer models. J Control Release 172:86-95|
Showing the most recent 10 out of 40 publications